A rapid method for the extraction and purification of DNA from human leukocytes

Autor:	Earle R. Nestmann, Ann F. Hanham, Andrew A. Potter
Rok vydání:	1985
Předmět:	chemistry.chemical_classification Cancer Research DNA ligase Lysis DNA Ligases biology Chemistry Ion chromatography DNA DNA extraction Chromatography DEAE-Cellulose chemistry.chemical_compound Oncology Biochemistry Leukocytes Nucleic acid biology.protein Humans Ribonuclease Ethanol precipitation
Zdroj:	Cancer Letters. 26:335-341
ISSN:	0304-3835
DOI:	10.1016/0304-3835(85)90058-8
Popis:	A rapid method for the extraction and purification of DNA from human leukocytes was developed. Crude nucleic acids were obtained by sodium docdecylsulfate (SDS) lysis and potassium acetate precipitation of other cellular material, and the DNA was purified by ribonuclease digestion, diethylaminoethyl (DEAE) cellulose chromatography and ethanol precipitation. DNA obtained by this method is biologically active as reflected by its ability to act as substrate for various nucleases and T4 DNA ligase. The yield was sufficiently high that DNA from less than 1 ml of blood could be used for a number of reactions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b1cc77e0a20801f835ad3d599e62f8e https://doi.org/10.1016/0304-3835(85)90058-8 Zobrazit plný text záznamu