Effects of inadequate vitamin E and/or selenium nutrition on the release of arachidonic acid metabolites in rat alveolar macrophages
| Autor: | William J. Scheuchenzuber, George Hildenbrandt, C. Channa Reddy, Mary Lou Eskew, John R. Burgess, Arian Zarkower, Richard W. Scholz |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Vitamin medicine.medical_specialty Thromboxane Metabolite medicine.medical_treatment Lipoxygenase Balanced salt solution Arachidonic Acids Biology Leukotriene B4 Biochemistry Dinoprostone Selenium chemistry.chemical_compound Endocrinology Internal medicine Hydroxyeicosatetraenoic Acids medicine Animals Vitamin E Vitamin E Deficiency Arachidonic Acid Macrophages Zymosan Rats Pulmonary Alveoli Thromboxane B2 medicine.anatomical_structure chemistry Prostaglandin-Endoperoxide Synthases Arachidonic acid Pulmonary alveolus Prostaglandin E |
| Zdroj: | Prostaglandins. 38:79-89 |
| ISSN: | 0090-6980 |
| Popis: | Effects of vitamin E and/or selenium (Se) deficiency on the secretion of arachidonic acid metabolites by zymosan-stimulated pulmonary alveolar macrophages (AM) were examined using cells from male Long-Evans hooded rats fed torula-yeast based diets with or without the supplementation of vitamin E (150 IU/kg) or Se (0.5 mg/kg). Alveolar macrophages obtained by lavage were purified by adherence and cultured for 4 h in Hankś balanced salt solution containing bovine serum albumin (0.1%) and zymosan (300 μg/ml). The arachidonic acid metabolites present in the culture supernatant were measured by radioimmunoassay. Altered vitamin E and Se nutrition had no effect on the number of cells or cell types recovered from the pulmonary airways. Alveolar macrophages derived from animals fed on diets deficient in vitamin E or Se or both nutrients secreted higher levels of prostaglandins E 2 and thromboxane B 2 . Levels of both 5-hydroxyeicosatetraenoic acid and leukotriene B 4 were significantly increased only in the group fed the diet adequate in Se but deficient in vitamin E. Our data suggest that vitamin E and Se might play an important role to control the levels of several physiologically and pathologically important arachidonic acid metabolites. |
| Databáze: | OpenAIRE |
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