CRISPR Editing Technology in Biological and Biomedical Investigation
Autor: | Kamel Khalili, Won-Bin Young, Rafal Kaminski, Pamela C. Roehm, Martyn K. White |
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Rok vydání: | 2017 |
Předmět: |
Gene Editing
0301 basic medicine Flexibility (engineering) Genetics Biomedical Research Genetic enhancement Cell Biology Computational biology Biology Biochemistry Genome Article 03 medical and health sciences 030104 developmental biology Genome editing Animals Humans CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Guide RNA Molecular Biology Gene Function (biology) |
Zdroj: | Journal of Cellular Biochemistry. 118:3586-3594 |
ISSN: | 1097-4644 0730-2312 |
Popis: | The CRISPR or clustered regularly interspaced short palindromic repeats system is currently the most advanced approach to genome editing and is notable for providing an unprecedented degree of specificity, effectiveness, and versatility in genetic manipulation. CRISPR evolved as a prokaryotic immune system to provide an acquired immunity and resistance to foreign genetic elements such as bacteriophages. It has recently been developed into a tool for the specific targeting of nucleotide sequences within complex eukaryotic genomes for the purpose of genetic manipulation. The power of CRISPR lies in its simplicity and ease of use, its flexibility to be targeted to any given nucleotide sequence by the choice of an easily synthesized guide RNA, and its ready ability to continue to undergo technical improvements. Applications for CRISPR are numerous including creation of novel transgenic cell animals for research, high-throughput screening of gene function, potential clinical gene therapy, and nongene-editing approaches such as modulating gene activity and fluorescent tagging. In this prospect article, we will describe the salient features of the CRISPR system with an emphasis on important drawbacks and considerations with respect to eliminating off-target events and obtaining efficient CRISPR delivery. We will discuss recent technical developments to the system and we will illustrate some of the most recent applications with an emphasis on approaches to eliminate human viruses including HIV-1, JCV and HSV-1 and prospects for the future. J. Cell. Biochem. 118: 3586-3594, 2017. © 2017 Wiley Periodicals, Inc. |
Databáze: | OpenAIRE |
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