Hydrogen Sulfide Upregulates Glutamate–Cysteine Ligase Catalytic Subunit, Glutamate–Cysteine Ligase Modifier Subunit, and Glutathione and Inhibits Interleukin-1β Secretion in Monocytes Exposed to High Glucose Levels
| Autor: | David Micinski, Laura Huning, Sushil K. Jain |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cell Survival
Glutamate-Cysteine Ligase Endocrinology Diabetes and Metabolism Protein subunit Interleukin-1beta Enzyme-Linked Immunosorbent Assay Monocytes chemistry.chemical_compound Downregulation and upregulation Catalytic Domain Internal Medicine medicine Humans Hydrogen Sulfide Inflammation chemistry.chemical_classification DNA ligase U937 cell business.industry GCLM Monocyte U937 Cells Original Articles Glutathione Atherosclerosis Molecular biology Up-Regulation Oxidative Stress Glucose medicine.anatomical_structure GCLC chemistry Immunology business |
| Zdroj: | Metabolic Syndrome and Related Disorders. 12:299-302 |
| ISSN: | 1557-8518 1540-4196 |
| Popis: | Glutathione (GSH) deficiency and interleukin-1β (IL-1β) upregulation are linked to the progression of vascular inflammation and atherosclerosis. The consumption of sulfide-rich vegetables is known to lower the risk of atherosclerosis. This study examined the hypothesis that hydrogen sulfide (H2S) upregulates the glutamate–cysteine ligase catalytic subunit (GCLC) and GSH and inhibits IL-1β in a monocyte cell model. U937 monocytes were supplemented with H2S (0–12.5 μM) for 2 hr and then exposed to a control or high glucose (HG, 25 mM) for 22 hr. Levels of GCLC and glutamate–cysteine ligase modifier subunit (GCLM) expression were determined by western blotting and GSH using high-performance liquid chromatography (HPLC), and IL-1β using enzyme-linked immunoassay (ELISA). H2S significantly (P |
| Databáze: | OpenAIRE |
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