Magnetic Resonance Angiography Reveals Increased Arterial Blood Supply and Tumorigenesis Following High Fat Feeding in a Mouse Model of Triple-negative Breast Cancer
| Autor: | Xiaobing Fan, Devkumar Mustafi, Marta Zamora, Rebecca Valek, Suzanne D. Conzen, Sully Fernandez, Matthew J. Brady, Michael Fitch, Gregory S. Karczmar, Victoria Werner, Erica Markiewicz, Jeffrey Mueller, Olufunmilayo I. Olopade |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Carcinogenesis Mammary gland Urology Blood volume Mammary Neoplasms Animal Triple Negative Breast Neoplasms Diet High-Fat Magnetic resonance angiography Article 030218 nuclear medicine & medical imaging 03 medical and health sciences Mice 0302 clinical medicine Breast cancer Imaging Three-Dimensional Mammary Glands Animal medicine Animals Radiology Nuclear Medicine and imaging Neoplasm Invasiveness Spectroscopy medicine.diagnostic_test business.industry Cancer Arteries Feeding Behavior Organ Size medicine.disease Tumor Burden Disease Models Animal medicine.anatomical_structure Tumor progression Regional Blood Flow Angiography Molecular Medicine Arterial blood Female business 030217 neurology & neurosurgery Magnetic Resonance Angiography |
| Zdroj: | NMR Biomed |
| Popis: | Breast cancer is the second most commonly diagnosed malignancy among women globally. Past magnetic resonance imaging (MRI) studies have linked a high animal fat diet (HAFD) to increased mammary cancer risk in the SV40Tag mouse model of triple-negative breast cancer. Here, serial MRI examines tumor progression and measures the arterial blood volume feeding mammary glands in low fat diet (LFD) or HAFD fed mice. Virgin female C3(1)SV40Tag mice (n=8), weaned at 3-weeks old, were assigned to a LFD (n=4, 3.7-kcal/g; 17.2% kcal from vegetable oil) or a HAFD (n=4, 5.3-kcal/g; 60% kcal from lard) group. From ages 8–12 weeks, weekly fast spin echo MR images and time-of-flight (TOF) MR angiography of inguinal mammary glands were acquired at 9.4T. Following in vivo MRI, mice were sacrificed. Inguinal mammary glands were excised and fixed for ex vivo MRI and histology. Tumor, blood and mammary gland volumes for each time point were measured from manually traced regions-of-interest; tumors were classified as invasive by histopathology-blinded observers. Our analysis confirmed a strong correlation between total tumor volume and blood volume in the mammary gland. Tumor growth rates from week 8–12 were 2-fold higher in HAFD-fed mice (0.42±0.14 week(−1)) than in LFD-fed mice (0.21±0.03 week(−1)), p |
| Databáze: | OpenAIRE |
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