Regulation of magnesium efflux from rat spleen lymphocytes
| Autor: | Arianna Di Francesco, Achille Cittadini, Valeria Covacci, Federica I. Wolf |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Antiporter Cell Indomethacin Biophysics chemistry.chemical_element 8-Bromo Cyclic Adenosine Monophosphate Stimulation Biochemistry Internal medicine medicine Extracellular Cyclic AMP Animals Cyclooxygenase Inhibitors Magnesium Lymphocytes Alprostadil Rats Wistar Molecular Biology Calcimycin Arachidonic Acid Ion Transport Ionophores Arachidonate metabolism Chemistry Sodium Interferon-alpha Rats Kinetics Endocrinology medicine.anatomical_structure Bucladesine Prostaglandins Rat Spleen Efflux Spleen Adenylyl Cyclases |
| Zdroj: | Archives of biochemistry and biophysics. 344(2) |
| ISSN: | 0003-9861 |
| Popis: | Rat spleen lymphocytes (RSL) incubated at 37 degrees C in Mg-free medium (O-trans conditions) exibited Mg2+ efflux with apparent velocity of 0.2 nmol/mg protein/min. After 30 min, this process accounted for the mobilization of about 15% of cell total Mg2+. Half of the Mg2+ efflux depended on extracellular Na+ and was stimulated by cAMP. IFN-alpha significantly enhanced Mg2+ efflux under O-trans conditions as well as in the presence of physiological extracellular Mg2+. Pretreatment of RSL with indomethacin completely abolished IFN-alpha-induced Mg2+ efflux, suggesting a crucial role for cyclooxygenase-dependent arachidonate metabolism. On the other hand, pretreatment of RSL with the PKA inhibitor (Rp)8-Br-cAMPS prevented IFN-alpha stimulation of Mg2+ efflux, indicating the involvement of cAMP. Consistently, both IFN-alpha and exogenous PGE1 increased cAMP from 50 to 125 pmol/mg protein. Altogether these results show that IFN-alpha stimulates Mg2+ efflux by activating arachidonate metabolism and synthesis of prostaglandins. By influencing adenylcyclase activity, PGEs can eventually promote cAMP-dependent Mg2+ efflux, possibly through the activity of a Na-Mg antiport. In RSL, therefore, magnesium movements can be under the control of IFN-alpha and, perhaps, of other cytokines, suggesting the involvement of Mg2+ in cell response to receptor-mediated stimuli. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect