Suppressive Effects of Selected Food Phytochemicals on CD74 Expression in NCI-N87 Gastric Carcinoma Cells
| Autor: | Kazuto Washida, Hirotaka Sekiguchi, Akira Murakami |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Clinical Biochemistry
Cell Medicine (miscellaneous) Chronic gastritis Nobiletin cell-based ELISA chemistry.chemical_compound medicine bergamottin Nutrition and Dietetics Helicobacter pylori biology business.industry Cancer medicine.disease biology.organism_classification Bergamottin adhesion medicine.anatomical_structure chemistry CD74 Immunology Cancer research Original Article business Quercetin Luteolin |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| DOI: | 10.3164/jcbn.2008054 |
| Popis: | Helicobacter pylori (H. pylori) is one of the most widespread human pathogens, and plays major roles in chronic gastritis and gastric cancer. CD74 of gastric epithelial cells has recently been identified as an adhesion molecule to urease in H. pylori. In this study, we found that CD74 is highly expressed in a constitutive manner in NCI-N87 human gastric carcinoma cells at both the protein and mRNA levels as compared with Hs738St./Int fetal gastric cells. Subsequently, a novel cell-based ELISA able to rapidly screen the suppressive agents of CD74 expression was established. NCI-N87 cells were treated separately with 25 different food phytochemicals (4-100 microM) for 48 h and subjected to our novel assay. From those results, a citrus coumarin, bergamottin, was indicated to be the most promising compound with an LC(50)/IC(50) value greater than 7.1, followed by luteolin (>5.4), nobiletin (>5.3), and quercetin (>5.1). Our findings suggest that these CD74 suppressants are unique candidates for preventing H. pylori adhesion and subsequent infection with reasonable action mechanisms. |
| Databáze: | OpenAIRE |
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