REGULATION OF FIBROGENESIS DURING THE EARLY PHASE OF COMMON BILE DUCT OBSTRUCTION
| Autor: | Sibel Dinçer, Şükrü Bozkurt, Leyla Memiş, S. Ercan, Neslihan Bukan, Atilla Engin |
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| Rok vydání: | 2006 |
| Předmět: |
Liver Cirrhosis
Male medicine.medical_specialty Common Bile Duct Diseases Guinea Pigs Dinoprostone Lipid peroxidation chemistry.chemical_compound Hydroxyproline Malondialdehyde Internal medicine medicine Animals Nitrites Cell Proliferation Cholestasis Nitrates Fibrous capsule of Glisson Common bile duct business.industry Bile duct General Medicine Anatomy Leukotriene C4 Disease Models Animal Endocrinology medicine.anatomical_structure Liver chemistry Disease Progression Hepatic stellate cell lipids (amino acids peptides and proteins) Surgery Collagen Hepatic fibrosis business |
| Zdroj: | ANZ Journal of Surgery. 76:645-648 |
| ISSN: | 1445-2197 1445-1433 |
| Popis: | Background: Both nitric oxide (NO) and prostaglandins have been proposed as inhibitor substances involved in collagen deposition in the hepatic parenchyma. The possible reciprocal connections between NO and eicosanoids in the development of liver fibrosis were investigated during the initial phase of common bile duct obstructions. Methods: A total of 30 male albino guinea pigs were randomly and equally assigned to three groups. Group 1 underwent sham laparotomy. Group 2 and group 3 were subjected to permanent common bile duct ligature for 24 and 72 h , respectively. Changes in the liver prostaglandin E2 (PGE2), leukotriene C4, malondialdehyde contents and plasma nitrite plus nitrate concentrations were measured. To evaluate the extent of hepatic fibrosis, histological assessment of liver was confirmed with the equivalent hydroxyproline contents of liver. Results: Twenty-four hours after ligature, the amount of malondialdehyde and PGE2 and plasma nitrite plus nitrate concentrations increased significantly, whereas liver hydroxyproline contents did not change. However, 72 h after ligature (Group 3), lipid peroxidation and collagen deposition were significantly higher than that of the group 2 animals. The PGE2 : leukotriene C4 ratio peaked at 24 h and later decreased, whereas PGE2 : NO ratio remained unchanged in both group 2 and group 3 animals. Conclusions: The initiation of collagen synthesis occurred in portal tract as early as within the first 72 h of bile duct obstruction. The optimum function of reactive oxygen species on the stellate cell activation might be determined by the interaction between NO and PGE2. |
| Databáze: | OpenAIRE |
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