Toxoplasma gondii in vegetables from fields and farm storage facilities in the Czech Republic
| Autor: | Radka Dziedzinska, Michal Slany, Petr Kralik, Iva Slana, Vladimir Babak, M. Moravkova |
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| Rok vydání: | 2019 |
| Předmět: |
Veterinary medicine
Irrigation Farms Food Safety Genotype Real-Time Polymerase Chain Reaction Microbiology Irrigation water 03 medical and health sciences Food Parasitology Vegetables Genetics Molecular Biology Genotyping Czech Republic 030304 developmental biology 0303 health sciences biology 030306 microbiology business.industry Significant difference Toxoplasma gondii biology.organism_classification Food safety Restriction fragment length polymorphism business Toxoplasma |
| Zdroj: | FEMS Microbiology Letters. 366 |
| ISSN: | 1574-6968 |
| Popis: | Infection with Toxoplasma gondii has usually been connected with consumption of improperly treated meat. However, contaminated water and products of plant origin have emerged as new sources of infection in the last few years. Here, 292 vegetable samples—carrot, cucumber and lettuce—obtained from nine farms in the Czech Republic were examined using triplex real time PCR targeting two specific T. gondii sequences. Irrigation water and water used for washing of vegetables were also included. Overall, a positivity rate of 9.6% was found in vegetables. The concentration varied between 1.31 × 100 and 9.00 × 102 oocysts/g of sample. A significant difference was found between the positivity of vegetables collected directly from fields and that of vegetables collected from farm storage rooms (4.4–8.6% vs 10–24.1%, respectively). All samples of irrigation water and water used to rinse vegetables were negative. Genotyping based on restriction fragment length polymorphism (RFLP) analysis using seven markers revealed the exclusive presence of genotype II. |
| Databáze: | OpenAIRE |
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