Characterising nutrient-induced fluorescence transients (NIFTs) in nitrogen-stressed Chlorella emersonii (Chlorophyta)
| Autor: | John Beardall, Tara Higgins, Philip Heraud, Donald McNaughton, Bayden R. Wood, Kirsten Shelly |
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| Rok vydání: | 2007 |
| Předmět: |
inorganic chemicals
medicine.diagnostic_test biology Photosystem II food and beverages Plant Science Chlorophyta Aquatic Science biology.organism_classification Photosynthesis Fluorescence Chlorella Pigment Biochemistry Spectrophotometry visual_art medicine Biophysics visual_art.visual_art_medium Chlorophyll fluorescence |
| Zdroj: | Monash University |
| ISSN: | 0031-8884 |
| Popis: | responses of cells to N-starvation and additions of both NH4 + and NO3 2 were characterised using a number of techniques including NIFTs, conventional pigment analysis using UV/Visible spectrophotometry and Fourier transform infrared (FT-IR) and Raman spectroscopy. Results showed that the additio no f NH 4 + and NO3 2 elicited distinct changes in in vivo chl a fluorescence in N-limited Chlorella cells. The nature of the fluorescence change was dependent on the form of nitrogen supplied, with NH4 + additions producing a more complex effect on fluorescence than NO3 2 additions. Interestingly, the greatest fluorescence response followin gN H 4 + injection occurred prior to cells becoming extremely N-starved. Despite Chlorella exhibiting many of the characteristic features of N-deficient cells such as lowered capacity for photosynthetic electron transport (rETRmax), reduced Chl a : b–carotene ratios and increased levels of carbohydrate accumulation relative to protein, values for the maximum effective quantum yield of Photosystem II (WPSIIe-max) remained high over the course of N-starvation. |
| Databáze: | OpenAIRE |
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