Controlled charged amino acids of Ti-binding peptide for surfactant-free selective adsorption
| Autor: | Mutsunori Uenuma, Yukiharu Uraoka, Megumi Fukuta, Ichiro Yamashita, Heiji Watanabe, Bin Zheng, Naofumi Okamoto |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Inorganic chemistry Lysine Static Electricity Polysorbates Peptide Surface-Active Agents Adsorption Colloid and Surface Chemistry Pulmonary surfactant Amino Acids Physical and Theoretical Chemistry chemistry.chemical_classification Titanium Chemistry Spectrum Analysis Substrate (chemistry) General Medicine Surfaces and Interfaces Chromatography Ion Exchange Silicon Dioxide Amino acid Selective adsorption Ferritins Peptides Protein adsorption Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 118:25-30 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2014.03.024 |
| Popis: | The adsorption mechanism of titanium-binding peptide (TBP) on metal oxide substrates was investigated by evaluating the adsorption behavior of ferritins with various alanine-substituted TBPs. Results revealed that (a) a positively charged amino acid, lysine (K) or arginine (R), in TBP can anchor ferritin to negative zeta-potential substrates, (b) the adsorption force of K is stronger than R, and (c) local electrostatic interactions and flexibility of TBP directly affect adsorption. Based on these findings, selective ferritin adsorption on SiO2 with TiOX patterned surfaces in a surfactant-free condition was demonstrated. Alanine-substituted TBP with one positively charged amino acid (K) and one negatively charged amino acid (D), achieved ferritin-selective adsorption without a surfactant. The importance of controlled electrostatic forces between TBP and a substrate for selective adsorption without a surfactant was clearly demonstrated. |
| Databáze: | OpenAIRE |
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