Receptor-binding domain of SARS-CoV-2 spike protein efficiently inhibits SARS-CoV-2 infection and attachment to mouse lung
| Autor: | Chonsaeng Kim, Bum-Tae Kim, Gi Uk Jeong, Insu Hwang, Gun Young Yoon, Kyun-Do Kim, Hyun Woo Moon, Keun Bon Ku, Seongjun Kim, Dae-Gyun Ahn, Hye Jin Shin, Sun-Hee Lee, Hae Soo Kim, Young-Chan Kwon, Sumin Lee |
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| Rok vydání: | 2021 |
| Předmět: |
anti-viral
Signal peptide Recombinant Fusion Proteins viruses Protein domain Virus Attachment Microbial Sensitivity Tests Biology medicine.disease_cause Applied Microbiology and Biotechnology law.invention Protein Domains law Chlorocebus aethiops therapeutics medicine Animals Humans Amino Acid Sequence Binding site skin and connective tissue diseases Vero Cells Molecular Biology Peptide sequence Administration Intranasal Ecology Evolution Behavior and Systematics Coronavirus Binding Sites SARS-CoV-2 fungi HEK 293 cells COVID-19 virus diseases Cell Biology Virology COVID-19 Drug Treatment respiratory tract diseases Mice Inbred C57BL HEK293 Cells Spike Glycoprotein Coronavirus Recombinant DNA Vero cell Female receptor-binding domain Research Paper Developmental Biology |
| Zdroj: | International Journal of Biological Sciences |
| ISSN: | 1449-2288 |
| Popis: | COVID-19, caused by a novel coronavirus, SARS-CoV-2, poses a serious global threat. It was first reported in 2019 in China and has now dramatically spread across the world. It is crucial to develop therapeutics to mitigate severe disease and viral spread. The receptor-binding domains (RBDs) in the spike protein of SARS-CoV and MERS-CoV have shown anti-viral activity in previous reports suggesting that this domain has high potential for development as therapeutics. To evaluate the potential antiviral activity of recombinant SARS-CoV-2 RBD proteins, we determined the RBD residues of SARS-CoV-2 using a homology search with RBD of SARS-CoV. For efficient expression and purification, the signal peptide of spike protein was identified and used to generate constructs expressing recombinant RBD proteins. Highly purified RBD protein fused with the Fc domain of human IgG showed potent anti-viral efficacy, which was better than that of a protein fused with a histidine tag. Intranasally pre-administrated RBD protein also inhibited the attachment of SARS-COV-2 to mouse lungs. These findings indicate that RBD protein could be used for the prevention and treatment of SARS-CoV-2 infection. |
| Databáze: | OpenAIRE |
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