An Integrated Approach to Gene Discovery and Marker Development in Atlantic Cod (Gadus morhua)
| Autor: | Cynthia Stone, Bruce A. Curtis, Stewart C. Johnson, Sharen Bowman, Marije Booman, Charles Y. Feng, Sophie Hubert, Jane E. Symonds, Jennifer R. Hall, Catherine Kozera, Matthew L. Rise, Brent Higgins, Marlies Rise, Gary Simpson, Jillian Tarrant Bussey, Tiago S. Hori, A. Kurt Gamperl, Jennifer Kimball, Edward A. Trippel, Tudor Borza |
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| Jazyk: | angličtina |
| Předmět: |
Animal production
Animal breeding Single-nucleotide polymorphism markers Gene discovery capture method expressed sequence tag Aquaculture EST collection Molecular marker Breeding Applied Microbiology and Biotechnology polymorphism single nucleotide polymorphism fishery management life cycle Gadus genetics selective breeding Bacteria (microorganisms) Elevated temperature Genetics overfishing Expressed Sequence Tags Expressed sequence tag biology ESTs fish protein gadoid methodology Genomics Conventional methods wild population Antigen stimulation Gadus morhua Response pathways Original Article genetic marker Alternative routes Integrated approach SNPs Biotechnology Fish Proteins Genetic Markers Genomics tools Canada Histology Aquaculture industry Fisheries DNA sequence Computational biology Monitoring tools Aquatic Science Selective breeding Polymorphism Single Nucleotide domestication Gene sequences Species Specificity Fisheries management gene expression profiling Animals Animalia gene library Selection Genetic Domestication Markers Tissue business.industry species difference Sequence Analysis DNA biology.organism_classification Life cycle stages Genes genetic selection Marker development Atlantic cod business metabolism |
| Zdroj: | Marine Biotechnology (New York, N.y.) |
| ISSN: | 1436-2228 |
| DOI: | 10.1007/s10126-010-9285-z |
| Popis: | Atlantic cod is a species that has been overexploited by the capture fishery. Programs to domesticate this species are underway in several countries, including Canada, to provide an alternative route for production. Selective breeding programs have been successfully applied in the domestication of other species, with genomics-based approaches used to augment conventional methods of animal production in recent years. Genomics tools, such as gene sequences and sets of variable markers, also have the potential to enhance and accelerate selective breeding programs in aquaculture, and to provide better monitoring tools to ensure that wild cod populations are well managed. We describe the generation of significant genomics resources for Atlantic cod through an integrated genomics/selective breeding approach. These include 158,877 expressed sequence tags (ESTs), a set of annotated putative transcripts and several thousand single nucleotide polymorphism markers that were developed from, and have been shown to be highly variable in, fish enrolled in two selective breeding programs. Our EST collection was generated from various tissues and life cycle stages. In some cases, tissues from which libraries were generated were isolated from fish exposed to stressors, including elevated temperature, or antigen stimulation (bacterial and viral) to enrich for transcripts that are involved in these response pathways. The genomics resources described here support the developing aquaculture industry, enabling the application of molecular markers within selective breeding programs. Marker sets should also find widespread application in fisheries management. Electronic supplementary material The online version of this article (doi:10.1007/s10126-010-9285-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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