Identification and evaluation of cell- growth-inhibiting bDtBPP-analogue degradation products from phosphite antioxidants used in polyolefin bioprocessing materials

Autor:	Elke Jurkiewicz, Roberto Menzel, Armin Hauk, Tanja Verena Maier, Helmar Görls, Dana Budde, Thomas Noll, Ina Pahl, Eric Täuscher
Rok vydání:	2020
Předmět:	Models Molecular Phosphites Antioxidant medicine.medical_treatment CHO Cells Polyenes 02 engineering and technology 01 natural sciences Biochemistry Antioxidants Analytical Chemistry chemistry.chemical_compound Cricetulus medicine Animals Humans Bioprocess Cytotoxicity Cell Proliferation Cell growth 010401 analytical chemistry 021001 nanoscience & nanotechnology Phosphate Combinatorial chemistry 0104 chemical sciences Polyolefin HEK293 Cells chemistry Cell culture Degradation (geology) 0210 nano-technology
Zdroj:	Analytical and Bioanalytical Chemistry. 412:4505-4518
ISSN:	1618-2650 1618-2642
DOI:	10.1007/s00216-020-02736-z
Popis:	The inhibiting effect of the secondary phosphite antioxidant degradation product bis(2,4-di-tert-butylphenyl)phosphate (bDtBPP) on cell growth is well-known. The present study describes structurally related compounds which are likely to be formed from similar widely used phosphite antioxidants used in materials for the manufacturing of single-use (SU) equipment. Two potential candidates of such compounds-3,3',5,5'-tetra-tert-butyl-2,2'-dihydroxybiphenylphosphate (TtBBP) and bis(p-nonylphenyl)phosphate (bNPP)-were identified by chromatography and mass spectrometry followed by synthesis and X-ray structure elucidation. Additionally, the formation of TtBBP was confirmed in an analytical degradation study and its migration from SU bioprocessing material was estimated. The cytotoxicity evaluation by means of cell culture spiking experiments and flow cytometry analysis revealed that' even if cell growth was inhibited by all the compounds to some extent, bDtBPP showed the most severe effect and stoods out from the other two degradants investigated. Graphical abstract.
Databáze:	OpenAIRE
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