Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer
| Autor: | Jinyu Gu, Chew Yee Ngan, Hirofumi Yamamoto, Ichiro Takemasa, Mitsugu Sekimoto, Minoru Ogawa, Morito Monden, Masataka Ikeda, Katsuki Danno, Naganori Kyo, Hideyuki Hemmi |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Angiogenesis Cell Culture Media Serum-Free Angiopoietin-2 Neovascularization Cell Line Tumor medicine Humans RNA Messenger Hypoxia Glucose Transporter Type 1 Oncogene Reverse Transcriptase Polymerase Chain Reaction business.industry Liver Neoplasms Cancer General Medicine Cell cycle Hypoxia (medical) HCT116 Cells medicine.disease Immunohistochemistry Cell Hypoxia Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Apoptosis cardiovascular system Cancer research medicine.symptom Colorectal Neoplasms business HT29 Cells hormones hormone substitutes and hormone antagonists |
| Zdroj: | Oncology Reports. |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.15.4.779 |
| Popis: | Angiogenesis is a compensatory mechanism that enables malignant tumors to survive in an oxygen-deficient environment. To test our hypothesis that hypoxia stimulates the production of angiopoietin-2 (Ang-2) in colorectal cancer (CRC), we investigated the expression of Ang-2 in three cultured CRC cell lines, and in specimens from 11 CRC metastatic liver tumors. Hypoxia-induced Ang-2 mRNA expression was clearly evident in HCT116 cells that did not express Ang-2 under normoxic conditions. Ang-2 mRNA was detected only after 48 h in hypoxic serum-deprived cultures in a LoVo cell line, and under both normoxic and hypoxic conditions without any noticeable difference in the HT29 cells. There was a stepwise increase in Ang-2 expression from the periphery to the central part of the liver metastatic foci, whereas an inverse result was noted in tumor blood vessels, with a gradual decrease in CD31-positive ECs from the edge to the central region of the metastatic lesion. An expression pattern similar to Ang-2 was found in glucose transporter 1 (Glut-1), a known hypoxia-induced factor. These findings suggest that hypoxia plays an important role in inducing the expression of Ang-2 in CRC. |
| Databáze: | OpenAIRE |
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