Hydrazine selective dual signaling chemodosimetric probe in physiological conditions and its application in live cells
| Autor: | Animesh Sahana, Yaroslav Filinchuk, Debasis Das, Ansuman Chatterjee, Maria G. Babashkina, Archya Sengupta, Sandip Nandi, Damir A. Safin, Nikolay Tumanov, Sandip Mandal |
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| Rok vydání: | 2015 |
| Předmět: |
Cell Survival
Hydrazine Molecular Conformation Crystallography X-Ray Photochemistry Biochemistry Analytical Chemistry Rhodamine Rhodamines chemistry.chemical_compound Nitriles Fluorescence microscope Humans Environmental Chemistry Spectroscopy Fluorescent Dyes Xanthene Fluorescence Benzonitrile Hydrazines Spectrometry Fluorescence Microscopy Fluorescence Xanthenes chemistry MCF-7 Cells Quantum Theory Conjugate |
| Zdroj: | Analytica Chimica Acta. 893:84-90 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2015.08.041 |
| Popis: | A rhodamine-cyanobenzene conjugate, (E)-4-((2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)ethylimino)methyl)benzonitrile (1), which structure has been elucidated by single crystal X-ray diffraction, was synthesized for selective fluorescent "turn-on" and colorimetric recognition of hydrazine at physiological pH 7.4. It was established that 1 detects hydrazine up to 58 nM. The probe is useful for the detection of intracellular hydrazine in the human breast cancer cells MCF-7 using a fluorescence microscope. Spirolactam ring opening of 1, followed by its hydrolysis, was established as a probable mechanism for the selective sensing of hydrazine. |
| Databáze: | OpenAIRE |
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