Conditions to optimise the developmental competence of immature equine oocytes
| Autor: | Robert Foss, Nancy L. Cook, David Battaglia, Richard Beck, Thomas O'Leary, Elizabeth S. Metcalf, Jeremy G. Thompson, Keith Masterson |
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| Rok vydání: | 2019 |
| Předmět: |
Sodium ascorbate
medicine.medical_treatment Embryonic Development Reproductive technology Fertilization in Vitro Biology Intracytoplasmic sperm injection Andrology chemistry.chemical_compound Endocrinology 1-Methyl-3-isobutylxanthine Genetics medicine Animals Blastocyst Horses Sperm Injections Intracytoplasmic Molecular Biology Forskolin Colforsin Embryo Oocyte In Vitro Oocyte Maturation Techniques medicine.anatomical_structure Reproductive Medicine chemistry Cell culture embryonic structures Oocytes Animal Science and Zoology Female Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 32(11) |
| ISSN: | 1031-3613 |
| Popis: | Optimising the developmental potential of immature equine oocytes and invitro-produced (IVP) embryos was explored through modifications of established media and holding temperature. In Experiment 1, delaying spontaneous resumption of meiosis through the process of simulated physiological oocyte maturation with the addition of the adenylate cyclase activator forskolin (50µM) and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (100µM) to overnight holding medium before maturation improved blastocyst production (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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