Molecular cloning and expression of a cyclic AMP activated chloride conductance regulator : a novel ATP-binding cassette transporter
| Autor: | Frans G. M. Russel, Peter M.T. Deen, M.A. van Kuijck, Andreas E. Busch, René J. M. Bindels, R.A.M.H. van Aubel, Florian Lang, C.H. van Os |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Cell Membrane Permeability
Patch-Clamp Techniques algemeen [Geneeskunde] Physiology Regulation of salt and water reabsorption in the renal collecting duct ATP-binding cassette transporter Kidney Chloride Physiology General (Non MeSH) Polymerase Chain Reaction General (Non MeSH) Epithelium Protein Structure Secondary Medische Wetenschappen Membrane Potentials Kidney Concentrating Ability Xenopus laevis Adenosine Triphosphate Adrenal Cortex Hormones 1-Methyl-3-isobutylxanthine Intestine Small Cloning Molecular Urinary Tract GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Cells Cultured Phylogeny Niet-toepassingsgericht onderzoek Multidisciplinary Cultured Hypothalamic Hormones Reabsorption Rol en vermeende dubbelfunctie van P-glycoproteïne als multifarmacontransporter en chloridekanaal in de nier Niflumic acid Niflumic Acid Gezondheid Geneeskunde: algemeen Water-Electrolyte Balance Cystic fibrosis transmembrane conductance regulator Recombinant Proteins Financial Economics Cell biology Biochemistry Regulatie water en zouttransport in de verzamelbuis van de nier Liver Organ Specificity Chloride channel Female Kidney Diseases Rabbits medicine.drug Research Article Logic Cells Molecular Sequence Data Urogenital System Biology Medical sciences Cell Physiology (Non MeSH) Chloride Channels medicine Animals Humans ATP Binding Cassette Transporter Subfamily B Member 1 Amino Acid Sequence Ion transporter Ion Transport Role and putative double function of P-glycoprotein as multidrug transporter and chloride channel in the kidney Sequence Homology Amino Acid Cell Membrane Colforsin Apical membrane Blotting Northern Hormones Models Structural biology.protein Oocytes ATP-Binding Cassette Transporters |
| Zdroj: | Proceedings of the National Academy of Sciences USA, 93, 5401-5406 Proceedings of the National Academy of Science USA, 93, 11, pp. 5401-5406 Proceedings of the National Academy of Science USA, 93, 5401-5406. Natl acad sciences Proceedings of the National Academy of Science USA, 93, 5401-5406 Proceedings of the National Academy of Science USA, 93, pp. 5401-5406 Proceedings of the National Academy of Sciences USA, 9, pp. 5401-5406 Proceedings of the National Academy of Sciences USA, 93, pp. 5401-5406 Proceedings of the National Academy of Sciences USA, 9, 5401-5406 |
| ISSN: | 0027-8424 |
| Popis: | Cystic fibrosis transmembrane conductance regulator (CFTR) is an ATP-regulated, cAMP-activated chloride channel located in the apical membrane of many epithelial secretory cells. Here we report cloning of a cAMP-activated epithelial basolateral chloride conductance regulator (EBCR) that appears to be a basolateral CFTR counterpart. This novel chloride channel or regulator shows 49% identity with multidrug resistance-associated protein (MRP) and 29% identity with CFTR. On expression in Xenopus oocytes, EBCR confers a cAMP-activated chloride conductance that is inhibited by the chloride channel blockers niflumic acid, 5-nitro-2-(3-phenylpropylamine)benzoic acid, and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid. Northern blot analysis reveals high expression in small intestine, kidney, and liver. In kidney, immunohistochemistry shows a conspicuous basolateral localization mainly in the thick ascending limb of Henle's loop, distal convoluted tubules and to a lesser extent connecting tubules. These data suggest that in the kidney EBCR is involved in hormone-regulated chloride reabsorption. |
| Databáze: | OpenAIRE |
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