Possible functional involvement of thymosin beta 4 in developing tooth germ of mouse lower first molar
| Autor: | Jun Ya Honda, Xie Ming, Hidetaka Sakai, Merina Akhter, Haruyoshi Yamaza, Ieyoshi Kobayashi, Hiroko Wada, Tamotsu Kiyoshima, Kou Matsuo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Cervical loop Biology Mice stomatognathic system medicine Animals RNA Messenger Dental Enamel Molecular Biology In Situ Hybridization reproductive and urinary physiology Reduced enamel epithelium Mice Inbred BALB C Inner enamel epithelium Enamel organ Tooth Germ Epithelial Cells Cell Biology Molar Dental lamina Epithelium Enamel knot Cell biology Thymosin Thymosin beta-4 stomatognathic diseases Medical Laboratory Technology medicine.anatomical_structure embryonic structures sense organs |
| Zdroj: | Histochemistry and Cell Biology. 124:207-213 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-005-0040-x |
| Popis: | We examined the detailed in situ expression pattern of thymosin beta 4 (Tbeta4) in the developing mouse mandibular first molar. Tbeta4 mRNA was expressed in the presumptive dental epithelium at embryonic day 10.5 (E10.5) and in the thickened dental epithelium at E12. An in situ signal was observed in the invaginated epithelial bud at E13, in the enamel organ at E14 and E14.5, and in the primary enamel knot (PEK) at E14.5. The signal was localized in the epithelial cells of the outer layer of the enamel organ at E15 and E15.5. No signal was found in the PEK at these stages. Tbeta4 mRNA was expressed in the inner enamel epithelium, cervical loop and dental lamina at E16 and E17. The expression of Tbeta4 mRNA was observed in the polarized inner epithelial cells at E18, newborn day 1 (N1) and N2. However, the signal intensity decreased markedly at N3. We herein report for the first time that Tbeta4 is distinctly expressed in developing tooth germ, and it may also play functional roles in the initiation, growth and differentiation of tooth germ. |
| Databáze: | OpenAIRE |
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