Endocytosis by liver cells during suppression of intralysosomal proteolysis
| Autor: | E. V. Rukavishnikova, T. A. Korolenko, Safina Af, Mynkina Gi, M. I. Dushkin |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Cathepsin H Proteolysis Phagocytosis Cathepsin L Cathepsin D Pharmacology Endocytosis Biochemistry Cathepsin B Polyethylene Glycols Chloroquine In vivo Endopeptidases medicine Animals Cathepsin medicine.diagnostic_test Chemistry Hydrolysis Macrophages Rats Inbred Strains Cathepsins In vitro Rats Cysteine Endopeptidases Liver Lysosomes medicine.drug |
| Zdroj: | Biological chemistry Hoppe-Seyler. 373(7) |
| ISSN: | 0177-3593 |
| Popis: | The lysosomotropic agent chloroquine is widely used as a specific inhibitor of intralysosomal proteolysis in isolated hepatocytes. It was shown that in vitro chloroquine reversibly inhibited purified cathepsins H, B, L in concentrations less than those observed inside lysosomes in vivo. However, administration of high doses of chloroquine to rats (30-50 mg/kg i.p. as a single or repeated injections) was followed by increased cathepsin D and cysteine proteinase activities, as well as other lysosomal enzymes. Chloroquine administration did not induce any changes of carbon particles phagocytosis by liver cells (macrophages); modifications of fluid-phase (125I-PVP uptake) and receptor-mediated endocytosis (125I-asialo-fetuin uptake) were noted. Chloroquine administered in vivo reproduced some symptoms of lysosomal storage diseases (especially during repeated drug administration). |
| Databáze: | OpenAIRE |
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