Fecal carriage of multidrug-resistant Escherichia coli by community children in southern Taiwan

Autor: Yun-Ju Hung, Ming-Fang Cheng, Jiun-Ling Wang, Wan-Yu Hung, Chih-Hsin Hung, Wen-Chi Chen, I-Fei Huang, Wei-Yang Lee, Hong-Hsiang Hu, Ying-Tso Shen
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Adolescent
medicine.drug_class
030106 microbiology
Antibiotics
Cefazolin
Taiwan
Microbial Sensitivity Tests
medicine.disease_cause
Microbiology
03 medical and health sciences
Feces
0302 clinical medicine
Fecal carriage
Extended-spectrum β-lactamase
Ampicillin
Drug Resistance
Multiple
Bacterial
medicine
Escherichia coli
Humans
Multidrug-resistant
030212 general & internal medicine
Prospective Studies
lcsh:RC799-869
Child
Children
Escherichia coli Infections
business.industry
Gastroenterology
Infant
Newborn
Infant
General Medicine
Bacterial Typing Techniques
Multiple drug resistance
Ciprofloxacin
Specimen collection
Child
Preschool
Carrier State
Multilocus sequence typing
lcsh:Diseases of the digestive system. Gastroenterology
business
medicine.drug
Research Article
Multilocus Sequence Typing
Zdroj: BMC Gastroenterology, Vol 18, Iss 1, Pp 1-8 (2018)
BMC Gastroenterology
DOI: 10.1186/s12876-018-0807-x
Popis: Background The emergence of multidrug-resistant (MDR) Escherichia coli (E. coli), particularly E. coli sequence type ST131, is becoming a global concern. Commensal bacteria, an important reservoir of antibiotic resistance genes, facilitate the spread of such genes to pathogenic bacterial strains. The objective of the study is to investigate the fecal carriage of MDR E. coli and ST131 E. coli in community children in Southern Taiwan. Methods In this prospective study, stool samples from children aged 0–18 years were obtained within 3 days of hospitalization from October 2013 to September 2014. Children with a history of underlying diseases, antibiotic treatment, or hospitalization in the 3 months before specimen collection were excluded. E. coli colonies were selected and tested for antimicrobial susceptibility, and O25b-ST131, multilocus sequence typing, and blaCTX-M gene groups were detected. Results Among 157 E. coli isolates, the rates of nonsusceptibility to ampicillin, amoxycillin + clavulanate, trimethoprim–sulfamethoxazole, and cefazolin were 70, 65.6, 47.1, and 32.5%, respectively. Twenty-nine (18.5%) isolates were nonsusceptible to ciprofloxacin. MDR E. coli accounted for 58 (37%) of all isolates. Thirteen (8.3%) isolates produced extended-spectrum β-lactamase (ESBL). Furthermore, 26 (16.6%) and 13 (8.3%) isolates were O25b and ST131 positive, respectively. Five (38.5%) of the 13 ESBL-producing E. coli belonged to blaCTX-M group 9, among which were CTXM-14 and 4 (80%) were O25b–ST131 positive. Compared with the non-ESBL and ciprofloxacin-susceptible groups, the ESBL and ciprofloxacin-nonsusceptible groups showed significantly higher rates of O25b–ST131 positivity. Conclusions The prevalence of the fecal carriage of nonsusceptible E. coli in children was high; among these E. coli, 37% were MDR, 18.5% were nonsusceptible to ciprofloxacin, and 8.3% produced ESBL. O25b–ST131 was the most common ESBL-producing E. coli clonal group present in the feces of children, and the ESBL and ciprofloxacin-nonsusceptible groups showed significantly higher rates of O25b–ST131 positivity. Electronic supplementary material The online version of this article (10.1186/s12876-018-0807-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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