A quantitative polymerase chain reaction assay for the detection of equine (horse and donkey)-originated meat in processed bovine meat products
| Autor: | Mehmet Inan, Semiramis Yılmaz, Sevda Gökbora, Aysun Türkanoǧlu Özçelik |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Meat food.ingredient General Chemical Engineering Horse meat Food Contamination Biology Polymerase Chain Reaction 01 natural sciences Industrial and Manufacturing Engineering Melting curve analysis 0404 agricultural biotechnology food 010608 biotechnology Food Quality Animals Species identification Horses Food science food and beverages Horse DNA Equidae 04 agricultural and veterinary sciences Consumer protection 040401 food science Meat Products Real-time polymerase chain reaction Cattle Donkey Food Analysis Food Science |
| Zdroj: | Food Science and Technology International. 25:38-46 |
| ISSN: | 1532-1738 1082-0132 |
| Popis: | Meat is one of the most important basic foodstuffs in human nutrition. Nowadays, adulteration and authenticity are common problems for meat products. Identification of meat species is important in terms of consumer protection and prevention of adulteration. There are different methods to determine adulteration of meat and meat products. These methods are histological controls, serological tests, and quantitative polymerase chain reaction. In this study, species identification and quantification analysis of meat and meat products were done by using horse-, donkey-, and bovine-specific primers with quantitative polymerase chain reaction method. Triple meat mixtures containing horse and donkey meat ranging from 0.1 to 50% levels were prepared within a bovine mixture for using species identification and quantification analysis. The method specificity was confirmed by melting curve analysis. In conclusion, quantitative polymerase chain reaction is an easy, rapid, and reliable method for meat species identification, and with this study an applicable method was developed for the detection and quantification of equine-originated meat in bovine meat products. |
| Databáze: | OpenAIRE |
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