A New Oxidative Stress Model, 2,2-Azobis(2-Amidinopropane) Dihydrochloride Induces Cardiovascular Damages in Chicken Embryo

Autor: Ruo-Nan Yi, Xiao-yu Wang, Xuesong Yang, Kenneth Ka Ho Lee, Keiichi Abe, Xiao-Di Li, Hiroshi Kurihara, Bun Tsoi, Rong-Rong He, Yan Li
Rok vydání: 2013
Předmět:
Embryology
Amidines
lcsh:Medicine
Chick Embryo
medicine.disease_cause
Cardiovascular System
Pediatrics
Chorioallantoic Membrane
Pregnancy
Pediatric Cardiology
Morphogenesis
lcsh:Science
chemistry.chemical_classification
Teratology
Multidisciplinary
Ventricular Remodeling
Obstetrics and Gynecology
Embryo
Oxidants
Chorioallantoic membrane
medicine.anatomical_structure
embryonic structures
Heart Development
Medicine
Research Article
animal structures
Neovascularization
Physiologic
Cardiomegaly
Biology
Models
Biological
Lethal Dose 50
Andrology
Vasculogenesis
medicine
Animals
Birth Defects
Yolk sac
Reactive oxygen species
lcsh:R
Embryogenesis
Pregnancy Complications
Oxidative Stress
chemistry
Immunology
lcsh:Q
Heart enlargement
Reactive Oxygen Species
Organism Development
Oxidative stress
Developmental Biology
Zdroj: PLoS ONE, Vol 8, Iss 3, p e57732 (2013)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0057732
Popis: It is now well established that the developing embryo is very sensitive to oxidative stress, which is a contributing factor to pregnancy-related disorders. However, little is known about the effects of reactive oxygen species (ROS) on the embryonic cardiovascular system due to a lack of appropriate ROS control method in the placenta. In this study, a small molecule called 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH), a free radicals generator, was used to study the effects of oxidative stress on the cardiovascular system during chick embryo development. When nine-day-old (stage HH 35) chick embryos were treated with different concentrations of AAPH inside the air chamber, it was established that the LD50 value for AAPH was 10 µmol/egg. At this concentration, AAPH was found to significantly reduce the density of blood vessel plexus that was developed in the chorioallantoic membrane (CAM) of HH 35 chick embryos. Impacts of AAPH on younger embryos were also examined and discovered that it inhibited the development of vascular plexus on yolk sac in HH 18 embryos. AAPH also dramatically repressed the development of blood islands in HH 3+ embryos. These results implied that AAPH-induced oxidative stress could impair the whole developmental processes associated with vasculogenesis and angiogenesis. Furthermore, we observed heart enlargement in the HH 40 embryo following AAPH treatment, where the left ventricle and interventricular septum were found to be thickened in a dose-dependent manner due to myocardiac cell hypertrophy. In conclusion, oxidative stress, induced by AAPH, could lead to damage of the cardiovascular system in the developing chick embryo. The current study also provided a new developmental model, as an alternative for animal and cell models, for testing small molecules and drugs that have anti-oxidative activities.
Databáze: OpenAIRE
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