Cytogenetic Analysis of Human Bile for Mutagenicity and Co-Mutagenicity
| Autor: | Hiroshi Mano, Masaharu Yamamoto, Masatomo Watanabe, Junko Magara, Shuko Takagi, Hiroto Nakadaira, Sadahiko Akai, Masashi Tsunoda, Kiyoshi Kato, Kazuo Endoh |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Mitomycin medicine.medical_treatment Biology Gastroenterology General Biochemistry Genetics and Molecular Biology Giemsa stain Mitomycins Internal medicine medicine Bile Humans Lymphocytes Saline Cells Cultured Chromosome Aberrations Staining and Labeling Mutagenicity Tests Stomach Mitomycin C General Medicine medicine.disease medicine.anatomical_structure Biliary tract Mutation Chromosome abnormality Cholecystitis Female Pancreas |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 163:255-261 |
| ISSN: | 1349-3329 0040-8727 |
| Popis: | WATANABE, M., TAKAGI, S., MAGARA, J., MANO, H., TSUNODA, M., NAKADAIRA, H., ENDOH, K., YAMAMOTO, M., KATO, K. and AKAI, S. Cytogenetic Analysis of Human Bile for Mutagenicity and Co-Mutagenicity. Tohoku J. Exp. Med., 1991, 163 (4), 255-261- Cytogenetic analysis was used to test whether or not human bile induced chromosome abnormalities in lymphocytes grown in culture. Bile was obtained from gallbladders resected for various reasons such as cholecystitis, cholelithiasis, polypus and cancers of the biliary tract, stomach and pancreas. After adding human bile to a final concentration of 25μl/ml or 12.5μl/ml, the culture medium was incubated at 37°C for 72hr. Air-dried slides were stained with conventional Giemsa and the numerical and structural chromosome abnormalities were scored. Positive and negative controls in terms of chromosome abnormalities were established by using 0.03μg/ml mitomycin C (MMC) and 0.9% normal saline, respectively. Cytogenetic analysis was successfully performed in 6 out of 10 bile samples (60.0%). Bile alone did not induce numerical or structural chromosome abnormalities. Structural abnormalities increased significantly in the 25μl/ml bile +0.03μg/ml MMC group, compared with the 0.03μg/ml MMC group: 36.0% vs. 20.7% in the chromatid-type gaps and breaks, 27.8% vs. 22.7% in the chromosome-type gaps and breaks, and 8.3% vs. 3.2% in the exchange-type abnormalities. It is likely that the interaction between bile and MMC is synergistic rather than additive. |
| Databáze: | OpenAIRE |
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