Neonatal hypomyelination by the herbicide 2,4-dichlorophenoxyacetic acid. Chemical and ultrastructural studies in rats
| Autor: | Ricardo Duffard, G. Garcia, Ana María Evangelista de Duffard, Lidia M. Lopez, Cintia Konjuh, Alicia Brusco |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty 2 4-Dichlorophenoxyacetic acid Blotting Western Biology Toxicology Body weight Protein expression chemistry.chemical_compound Myelin Western blot Microscopy Electron Transmission Pregnancy Internal medicine medicine Image Processing Computer-Assisted Animals Lactation Rats Wistar Postnatal day Myelin Sheath medicine.diagnostic_test Herbicides Body Weight Malnutrition Neurotoxicity Brain medicine.disease Animals Suckling Rats Disease Models Animal Endocrinology medicine.anatomical_structure chemistry Maternal Exposure Ultrastructure Female 2 4-Dichlorophenoxyacetic Acid Injections Intraperitoneal Demyelinating Diseases |
| Zdroj: | Toxicological sciences : an official journal of the Society of Toxicology. 104(2) |
| ISSN: | 1096-0929 |
| Popis: | The purpose of this study was to determine whether 2,4-dichlorophenoxyacetic acid (2,4-D), which is an herbicide used to control the growth of broadleaf weeds, had a direct or an indirect (mediated by undernutrition) hypomyelinating effect. We also proposed to analyze the effect of 2,4-D on undernourished (UN) pups. Four experimental rat groups were used: well-nourished (WN) pups, litters with eight offsprings; UN pups, litters with fourteen offsprings; WN pups whose mother received 70 mg/kg/day of 2,4-D from postnatal day (PND) 9 to 21 (WN70 pups); and UN pups whose mother received 70 mg/kg/day of 2,4-D from PND 9 to 21 (UN70 pups). In this work, we demonstrated that (1) myelin proteins (analyzed by Western blot and/or immunohistochemical study) showed a significant decrease in WN70, UN, and UN70 with respect to control group; (2) there is a good correlation between these myelin-specific protein expression with the degree of myelin compaction detected by electron microscopy in groups exposed to 2,4-D; (3) a decreased and normal number of myelin sheets were detected in UN and 2,4-D exposed pups, respectively; and (4) undernourishment sensitized pups to the hypomyelinating effect of 2,4-D. According to this and besides the fact that WN70 group have no body weight changes, these results are indicating that 2,4-D and undernourishment are two independent hypomyelinating factors. |
| Databáze: | OpenAIRE |
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