Deficiency of T-Cell Intracellular Antigen 1 in Murine Embryonic Fibroblasts Is Associated with Changes in Mitochondrial Morphology and Respiration
| Autor: | José M. Izquierdo, Beatriz Ramos Velasco, Isabel Carrascoso |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Fundación Ramón Areces, Banco Santander |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
TIA1
Bioenergetics QH301-705.5 Cell Cell Respiration Mitochondrion Biology Mitochondrial Dynamics Catalysis Article Inorganic Chemistry Mice mitochondrial respiration Gene expression medicine Animals Physical and Theoretical Chemistry Biology (General) Molecular Biology mitochondria mitochondrial dynamics murine embryonic fibroblast QD1-999 Spectroscopy Mice Knockout Murine embryonic fibroblast Organic Chemistry General Medicine Fibroblasts Embryo Mammalian Embryonic stem cell Computer Science Applications Cell biology Mitochondria T-Cell Intracellular Antigen-1 Mice Inbred C57BL Crosstalk (biology) Chemistry medicine.anatomical_structure Mitochondrial dynamics Energy Metabolism Intracellular Mitochondrial respiration |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 12775, p 12775 (2021) Digital.CSIC. Repositorio Institucional del CSIC instname International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12775 |
| ISSN: | 1422-0067 |
| Popis: | T-cell intracellular antigen 1 (TIA1) is a multifunctional RNA-binding protein involved in regulating gene expression and splicing during development and in response to environmental stress, to maintain cell homeostasis and promote survival. Herein, we used TIA1-deficient murine embryonic fibroblasts (MEFs) to study their role in mitochondria homeostasis. We found that the loss of TIA1 was associated with changes in mitochondrial morphology, promoting the appearance of elongated mitochondria with heterogeneous cristae density and size. The proteomic patterns of TIA1-deficient MEFs were consistent with expression changes in molecular components related tomitochondrial dynamics/organization and respiration. Bioenergetics analysis illustrated that TIA1 deficiency enhances mitochondrial respiration. Overall, our findings shed light on the role of TIA1 in mitochondrial dynamics and highlight a point of crosstalk between potential pro-survival and pro-senescence pathways Ministerio de Economía y Competitividad (BFU2014-57735-R) and Ministerio de Ciencia e Innovación and Agencia Española de Investigación through FEDER funds (RTI2018-098517-B-100) (MICINN/AEI/FEDER, UE). The CBMSO receives an institutional grant from Fundación Ramón Areces and Banco Santander |
| Databáze: | OpenAIRE |
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