Proteolytic Activation ofVibrio mimicus(Vm) Major Outer Membrane Protein Haemagglutinin (HA) with Vm-HA/Protease: Implication for Understanding Bacterial Adherence
| Autor: | Kabir Uddin Ahmed, Munirul Alam, Sumio Shinoda, Shin Ichi Miyoshi, Nur A. Hasan, Ken Ichi Tomochika |
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| Rok vydání: | 2006 |
| Předmět: |
Proteases
Proteolysis medicine.medical_treatment Immunology Microbiology Bacterial Adhesion Vibrio mimicus Enzyme activator Virology medicine Extracellular Animals Protease biology medicine.diagnostic_test Metalloendopeptidases biology.organism_classification Enzyme Activation Hemagglutinins Biochemistry Cattle Bacterial outer membrane Bacteria Bacterial Outer Membrane Proteins |
| Zdroj: | Microbiology and Immunology. 50:845-850 |
| ISSN: | 0385-5600 |
| Popis: | Vibrio mimicus (Vm) haemagglutinins (HAs), such as an extracellular HA/protease (Vm-HA/protease) and a major outer membrane protein-HA (Vm-OMPHA), have been recognized as the putative adherence factors for the bacterium. However, the mechanism by which HAs coordinate the adherence function of the bacterium remains as yet unknown. We report herein the positive interaction between Vm-HA/protease and Vm-OMPHA resulting in significant enhancement of the haemagglutinating ability. In this interaction, no cleaved polypeptide was detected; however, limited proteolysis of Vm-OMPHA was confirmed by SDS-PAGE. The proteolytic activation of the native cell-associated Vm-OMPHA by limited proteolysis was also demonstrated in several V. mimicus strains. Proteolytic activation of OMPHA was also achieved with various proteases from bacterial and eukaryotic sources. These findings may indicate a novel coordination of V. mimicus HAs in the adherence of the bacterium. |
| Databáze: | OpenAIRE |
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