Microsatellite Primers for Two Threatened Orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)
| Autor: | Samantha A. N. Ferguson, Kelsey A. Peeples, Joanna Weremijewicz, Stephen H. Lu, Vanessa S. Hilaire, Danielle T. Palow, Catherine M. Martinez, Andrew B. Reeve, Jasmin I. Almonte, Carolina Valdes, Jason L. Downing, Michael Hass, Natalie M. Leon, Nicolas Z. Petrakis, Frank D. Lopez, Edson A. Zarate, Sarah M. Marrero, Ana K. Lam, Ebony D. Taylor |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetic diversity Orchidaceae biology commercially exploited plants Cyrtopodium punctatum Endangered species Plant Science Primer Note biology.organism_classification Encyclia tampensis microsatellites native Florida orchids 03 medical and health sciences 030104 developmental biology Natural population growth Genetic marker Botany Threatened species Microsatellite Ecology Evolution Behavior and Systematics |
| Zdroj: | Applications in Plant Sciences |
| ISSN: | 2168-0450 |
| DOI: | 10.3732/apps.1500095 |
| Popis: | Premise of the study: The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami’s urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae). Methods and Results: Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity (Ho) of 0.314 and average expected heterozygosity (He) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average Ho of 0.35 and an average He of 0.224. For C. punctatum, allele numbers ranged from one to three, with an average Ho of 0.257 and an average He of 0.272. Conclusions: These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project. |
| Databáze: | OpenAIRE |
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