Experimental bile-duct ligation resulted in accumulation of oxidized low-density lipoproteins in BALB/c mice liver
| Autor: | Mustafa Comert, Ishak Ozel Tekin, Yucel Ustundag, Vildan Sumbuloglu, Serefden Acikgoz, Zeki Acun, Bulent Hamdi Ucan, Figen Barut |
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| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi |
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Obstructive jaundice medicine.disease_cause BALB/c Lipid peroxidation chemistry.chemical_compound Mice Liver Function Tests Internal medicine Malondialdehyde medicine Animals Oxidized low-density lipoprotein Ligation Mice Inbred BALB C Hepatology medicine.diagnostic_test biology business.industry Gastroenterology Glutathione biology.organism_classification Lipoproteins LDL Jaundice Obstructive Endocrinology chemistry Biochemistry Liver Microscopy Fluorescence Biliary tract Bile Ducts Liver function tests business Oxidative stress Lipoprotein |
| Zdroj: | Journal of gastroenterology and hepatology. 19(9) |
| ISSN: | 0815-9319 |
| Popis: | Background and Aim: Oxidized low-density lipoproteins (LDL), which are produced during oxidative stress by the process of lipid peroxidation, have also been proposed to have complex roles in many other immuno-inflammatory mechanisms. It has been shown that bile-duct ligation results in oxidative stress in the liver of animals. The aim of this study was to investigate if oxidized LDL are produced in the liver tissues of bile-duct-ligated mice. Methods: Obstructive jaundice was induced in BALB/c mice by the ligation and division of the common bile duct. Liver concentrations of glutathione and malondialdehyde were measured in the sham-operated (n = 10) and bile-duct-ligated (n = 10) mice on the 10th day of obstructive jaundice. The presence of oxidized LDL in the liver tissue sections was evaluated using a special, novel immunofluorescent staining method. The final step was to explore the existence of oxidized LDL under fluorescent microscopy. Results: Compared with sham-operated mice, jaundiced mice showed significantly higher levels of malondialdehyde and lower concentrations of reduced glutathione in the liver. While there was no staining in the sham-operated group, bile-duct ligation resulted in positive oxidized LDL staining in the liver tissues of mice. The present study testifies that bile-duct ligation results in oxidative stress and enhanced lipid peroxidation in the hepatic tissues of BALB/c mice and moreover, that oxidized LDL accumulate in the liver of mice with experimental obstructive jaundice. Conclusion: Oxidized LDL may be an important and direct indicator of ongoing oxidative stress and enhanced lipid peroxidation in obstructive jaundice. The potential roles of this finding were also discussed, briefly. © 2004 Blackwell Publishing Asia Pty Ltd |
| Databáze: | OpenAIRE |
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