Characterization of the CD8+ T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice
| Autor: | Patricia M. A. de Graaff, Mireille Toebes, Mariska E. A. van Dijk, Ton N. Schumacher, Erwin A.W. Claassen, Grada M. van Bleek, Jan L. L. Kimpen, Peter Hoogerhout, Michaël V. Lukens, Robbert van der Most |
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| Přispěvatelé: | Strategic Infection Biology, Dep Infectieziekten Immunologie |
| Rok vydání: | 2006 |
| Předmět: |
C57BL/6
interferon-alpha Secondary infection T cell Molecular Sequence Data Antigen presentation Respiratory Syncytial Virus Infections CD8-Positive T-Lymphocytes Respiratory syncytial virus CD8+ T cells Inactivation susceptibility Epitope Cell Line Geneeskunde Mice Viral Proteins Immune system vaccine Virology fusion protein medicine Animals Humans Cytotoxic T cell Amino Acid Sequence inbred mice balb/c mice biology Scheikunde biology.organism_classification tract Specific Pathogen-Free Organisms Mice Inbred C57BL Disease Models Animal antigen presentation medicine.anatomical_structure ASG Infectieziekten Respiratory Syncytial Virus Human Immunology identification Female Peptides eosinophilia Tetramer CD8 |
| Zdroj: | Virology, 352(1), 157-168 FEBS letters, 580(1), 115 Virology 352 (2006) 1 |
| ISSN: | 0042-6822 0014-5793 |
| DOI: | 10.1016/j.virol.2006.04.023 |
| Popis: | The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4+ and CD8+ T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/ 6 background, we characterized the specificity, level and functional capabilities of CD8+ T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8+ T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice. © 2006 Elsevier Inc. All rights reserved. Keywords: Respiratory syncytial virus; C57BL/6; CD8+ T cells; Inactivation; Tetramer |
| Databáze: | OpenAIRE |
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