Intimate adhesion of Neisseria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator
Autor: | Ala-Eddine Deghmane, Mireille Larribe, Dario Giorgini, Muhamed-Kheir Taha, Stéphanie Petit, Yannick Pereira, Andrzej Topilko |
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Přispěvatelé: | Neisseria, Institut Pasteur [Paris], Station centrale de microscopie électronique (SCME), This work was supported by the Institut Pasteur., Institut Pasteur [Paris] (IP) |
Jazyk: | angličtina |
Rok vydání: | 2000 |
Předmět: |
Molecular Sequence Data
Mutant Regulator Neisseria meningitidis MESH: Base Sequence MESH: Meningococcal Infections Bacterial Adhesion General Biochemistry Genetics and Molecular Biology MESH: Neisseria meningitidis Cell Line 03 medical and health sciences Bacterial Proteins Transcriptional regulation Humans MESH: Bacterial Adhesion Molecular Biology Gene Transcription factor MESH: Bacterial Proteins 030304 developmental biology 0303 health sciences intimate adhesion LysR-regulator MESH: Gene Expression Regulation Bacterial MESH: Humans MESH: Molecular Sequence Data Base Sequence General Immunology and Microbiology biology 030306 microbiology General Neuroscience Epithelial Cells Promoter Articles Gene Expression Regulation Bacterial MESH: Transcription Factors biology.organism_classification Molecular biology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Cell Line Meningococcal Infections Cell culture MESH: Epithelial Cells Neisseria Transcription Factors |
Zdroj: | EMBO Journal EMBO Journal, EMBO Press, 2000, 19 (5), pp.1068-1078. ⟨10.1093/emboj/19.5.1068⟩ EMBO Journal, 2000, 19 (5), pp.1068-1078. ⟨10.1093/emboj/19.5.1068⟩ |
ISSN: | 0261-4189 1460-2075 |
Popis: | International audience; PilC1, a pilus-associated protein in Neisseria menin- gitidis, is a key element in initial meningococcal adhesion to target cells. A promoter element (CREN, contact regulatory element of Neisseria) is responsible for the transient induction of this gene upon cell contact. crgA (contact-regulated gene A) encodes a transcriptional regulator whose expression is also induced upon cell contact from a promoter region similar to the CREN of pilC1. CrgA shows significant sequence homologies to LysR-type transcriptional regulators. Its inactivation in meningococci provokes a dramatic reduction in bacterial adhesion to epithelial cells. Moreover, this mutant is unable to undergo intimate adhesion to epithelial cells or to provoke effacing of microvilli on infected cells. Purified CrgA is able to bind to pilC1 and crgA promoters, and CrgA seems to repress the expression of pilC1 and crgA. Our results support a dynamic model of bacteria-cell interaction involving a network of regulators acting in cascade. CrgA could be an intermediate regulator in such a network. |
Databáze: | OpenAIRE |
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