Intimate adhesion of Neisseria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator

Autor: Ala-Eddine Deghmane, Mireille Larribe, Dario Giorgini, Muhamed-Kheir Taha, Stéphanie Petit, Yannick Pereira, Andrzej Topilko
Přispěvatelé: Neisseria, Institut Pasteur [Paris], Station centrale de microscopie électronique (SCME), This work was supported by the Institut Pasteur., Institut Pasteur [Paris] (IP)
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Molecular Sequence Data
Mutant
Regulator
Neisseria meningitidis
MESH: Base Sequence
MESH: Meningococcal Infections
Bacterial Adhesion
General Biochemistry
Genetics and Molecular Biology
MESH: Neisseria meningitidis
Cell Line
03 medical and health sciences
Bacterial Proteins
Transcriptional regulation
Humans
MESH: Bacterial Adhesion
Molecular Biology
Gene
Transcription factor
MESH: Bacterial Proteins
030304 developmental biology
0303 health sciences
intimate adhesion
LysR-regulator
MESH: Gene Expression Regulation
Bacterial
MESH: Humans
MESH: Molecular Sequence Data
Base Sequence
General Immunology and Microbiology
biology
030306 microbiology
General Neuroscience
Epithelial Cells
Promoter
Articles
Gene Expression Regulation
Bacterial
MESH: Transcription Factors
biology.organism_classification
Molecular biology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Cell Line
Meningococcal Infections
Cell culture
MESH: Epithelial Cells
Neisseria
Transcription Factors
Zdroj: EMBO Journal
EMBO Journal, EMBO Press, 2000, 19 (5), pp.1068-1078. ⟨10.1093/emboj/19.5.1068⟩
EMBO Journal, 2000, 19 (5), pp.1068-1078. ⟨10.1093/emboj/19.5.1068⟩
ISSN: 0261-4189
1460-2075
Popis: International audience; PilC1, a pilus-associated protein in Neisseria menin- gitidis, is a key element in initial meningococcal adhesion to target cells. A promoter element (CREN, contact regulatory element of Neisseria) is responsible for the transient induction of this gene upon cell contact. crgA (contact-regulated gene A) encodes a transcriptional regulator whose expression is also induced upon cell contact from a promoter region similar to the CREN of pilC1. CrgA shows significant sequence homologies to LysR-type transcriptional regulators. Its inactivation in meningococci provokes a dramatic reduction in bacterial adhesion to epithelial cells. Moreover, this mutant is unable to undergo intimate adhesion to epithelial cells or to provoke effacing of microvilli on infected cells. Purified CrgA is able to bind to pilC1 and crgA promoters, and CrgA seems to repress the expression of pilC1 and crgA. Our results support a dynamic model of bacteria-cell interaction involving a network of regulators acting in cascade. CrgA could be an intermediate regulator in such a network.
Databáze: OpenAIRE
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