Omentin-1 alleviate interleukin-1β(IL-1β)-induced nucleus pulposus cells senescence

Autor:	Xin Huang, Changhong Chen, Yaofei Chen, Jun Xu, Lin Liu
Rok vydání:	2022
Předmět:	Nucleus Pulposus Sirtuin 1 Lectins Interleukin-1beta Cytokines Humans Bioengineering Intervertebral Disc Degeneration General Medicine GPI-Linked Proteins Applied Microbiology and Biotechnology Cellular Senescence Biotechnology
Zdroj:	Bioengineered. 13:13849-13859
ISSN:	2165-5987 2165-5979
DOI:	10.1080/21655979.2022.2084495
Popis:	One of the main causes of low back pain (LBP) and degenerative musculoskeletal disorders is intervertebral disc degeneration (IVDD). Inflammation-associated senescence of Human nucleus pulposus cells (HNPCs) plays an essential function in the disease progression of IVDD. Omentin-1 is an adipokine that has been recently reported to have anti-inflammatory potential. In our research, IL-1β was used to simulate the inflammatory environment in the IVDD. We investigated in vitro the effects of Omentin-1 on HNPCs, including the components of senescence, cell cycle and extracellular matrix (ECM) synthesis. The results showed that the addition of Omentin-1 improved IL-1β-induced senescence in HNPCs. G1 phase cell cycle arrest and reduced ECM synthesis in HNPCs. Furthermore, we demonstrated that the effect of Omentin-1 in reducing senescence of HNPCs is dependent on SIRT1. These findings suggest that Omentin-1 plays an important function in protecting HNPCs against senescence and has the potential for IVDD gene target therapy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3adba2a308e9fbb16251a9b909b66e08 https://doi.org/10.1080/21655979.2022.2084495 Zobrazit plný text záznamu