Symbiosis disruption in the olive fruit fly, Bactrocera oleae (Rossi), as a potential tool for sustainable control

Autor: Francesco Vinale, Francesco Pennacchio, Antonio P. Garonna, David Giron, Annie Bézier, Stefania Laudonia, Martina Sinno
Přispěvatelé: Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Napoli Federico II, Dipartimento di Agraria, University of Sassari, University of Naples Federico II, Sinno, Martina, Bézier, Annie, Vinale, Francesco, Giron, David, Laudonia, Stefania, Garonna, Antonio Pietro, Pennacchio, Francesco, Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2020
Předmět:
0106 biological sciences
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Olive fruit fly
01 natural sciences
Symbiosis
Olea
[SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture
Tephritidae
Animals
Bioassay
Bactrocera
Drosophila
Olive fruit fly
Symbiotic control
Trichoderma
Endosymbiotic bacteria
Secondary fungal metabolites
Sustainable control
Bactrocera oleae
bacterial quantification
qPCR
endosymbiotic bacteria
insect-microbes interactions
ComputingMilieux_MISCELLANEOUS
biology
business.industry
fungi
Pest control
General Medicine
biology.organism_classification
Biotechnology
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
010602 entomology
Fruit
Insect Science
Erwinia
Female
business
Agronomy and Crop Science
010606 plant biology & botany
Symbiotic bacteria
Zdroj: Pest Management Science
Pest Management Science, Wiley, 2020, ⟨10.1002/ps.5875⟩
Pest Management Science, Wiley, 2020, 76 (9), pp.3199-3207. ⟨10.1002/ps.5875⟩
ISSN: 1526-4998
1526-498X
Popis: Background The olive fruit fly Bactrocera oleae (Rossi) (OLF) is a major agricultural pest, whose control primarily relies on the use of chemical insecticides. Therefore, development of sustainable control strategies is highly desirable. The primary endosymbiotic bacterium of OLF, 'Candidatus Erwinia dacicola', is essential for successful larval development in unripe olive fruits. Therefore, targeting this endosymbiont with antimicrobial compounds may result in OLF fitness reduction and may exert control on natural populations of OLF. Results Here, we evaluate the impact of compounds with antimicrobial activity on the OLF endosymbiont. Copper oxychloride (CO) and the fungal metabolite viridiol (Vi), produced by Trichoderma spp., were used. Laboratory bioassays were carried out to assess the effect of oral administration of these compounds on OLF fitness and molecular analyses (quantitative polymerase chain reaction) were conducted to measure the load of OLF-associated microorganisms in treated flies. CO and Vi were both able to disrupt the symbiotic association between OLF and its symbiotic bacteria, determining a significant reduction in the endosymbiont and gut microbiota load as well as a decrease in OLF fitness. CO had a direct negative effect on OLF adults. Conversely, exposure to Vi significantly undermined larval development of the treated female's progeny but did not show any toxicity in OLF adults. Conclusions These results provide new insights into the symbiotic control of OLF and pave the way for the development of more sustainable strategies of pest control based on the use of natural compounds with antimicrobial activity. © 2020 Society of Chemical Industry.
Databáze: OpenAIRE
Externí odkaz: