Sustained alterations in neuroimmune gene expression after daily, but not intermittent, alcohol exposure
| Autor: | Terrence Deak, Tamara L. Doremus-Fitzwater, Anny Gano |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Neuroimmunomodulation Gene Expression Alpha (ethology) Hippocampus Article Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Corticosterone Internal medicine medicine Animals Molecular Biology Ethanol Chemistry General Neuroscience Brain Septal nuclei Interleukin Amygdala Rats IκBα Stria terminalis 030104 developmental biology Endocrinology medicine.anatomical_structure Immunology Cytokines Septal Nuclei Tumor necrosis factor alpha Neurology (clinical) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research. 1646:62-72 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2016.05.027 |
| Popis: | Acute ethanol intoxication is associated with Rapid Alterations in Neuroimmune Gene expression (RANGE), including increased Interleukin (IL)-6 and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα), and suppressed IL-1β and Tumor necrosis factor (TNF) α, yet little is known about adaptations in cytokines across the first few ethanol exposures. Thus, the present studies examined central cytokines during intoxication (3 h post-ethanol) following 2, 4 or 6 intragastric ethanol challenges (4 g/kg) delivered either daily or every-other-day (EOD). Subsequent analyses of blood ethanol concentrations (BECs) and corticosterone were performed to determine whether the schedule of ethanol delivery would alter the pharmacokinetics of, or general sensitivity to, subacute ethanol exposure. As expected, ethanol led to robust increases in IL-6 and IκBα gene expression in hippocampus, amygdala and bed nucleus of the stria terminalis (BNST), whereas IL-1β and TNFα were suppressed, thereby replicating our prior work. Ethanol-dependent increases in IL-6 and IκBα remained significant in all structures—even after 6 days of ethanol. When these doses were administered EOD, modest IL-6 increases in BNST were observed, with TNFα and IL-1β suppressed exclusively in the hippocampus. Analysis of BECs revealed a small but significant reduction in ethanol after 4 EOD exposures — an effect which was not observed when ethanol was delivered after 6 daily intubations. These findings suggest that ethanol-induced RANGE effects are not simply a function of ethanol load per se, and underscore the critical role that ethanol dosing interval plays in determining the neuroimmune consequences of alcohol. |
| Databáze: | OpenAIRE |
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