Scanning electron microscopy study of bone intracortical vessels using an injection and fractured surfaces technique
Autor: | Ugo E. Pazzaglia, Federico Ranchetti, Terenzio Congiu, Matteo Salari, Carlo Dell'Orbo |
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Rok vydání: | 2009 |
Předmět: |
Male
Materials science Scanning electron microscope Bone Matrix Intracortical vessels Matrix (biology) Bone remodeling Microcirculation Bone remodelling Cortex (anatomy) medicine Animals Femur Bone vessels non-myelinated nerve fibres Cutting cone General Medicine Anatomy Osteon Intracortical canals system Haversian System medicine.anatomical_structure Microscopy Electron Scanning Cortical bone Rabbits |
Zdroj: | Anatomical Science International. 85:31-37 |
ISSN: | 1447-073X 1447-6959 |
Popis: | The intracortical canal/vessel systems of long bones are not yet completely understood in terms of their morphology and physiology, mainly because of the difficulty of injecting the small calibre vessels and cutting the calcified matrix. Here, we apply a novel method combining perfusion of the vessels and fracture of the cortical bone to enlighten the architecture of this system. The femurs of ten rabbits were perfused with a water-soluble dye (China ink) or alcoholic glycerol solution, and the fractured cortex specimens were then examined by scanning electron microscopy (SEM). The results document: (1) the fibrillar structure of the canal surfaces; (2) the perivascular environment with cellular components in different phases of incorporation within the bone matrix; (3) previously unreported filamentous structures on the outer surface of vessels, which could be interpreted as non-myelinic nerve fibres; (4) the inner organisation of the cutting cones. Although based exclusively on morphology, these observation have some relevance to increasing knowledge of bone circulation physiology in the cortical bone. |
Databáze: | OpenAIRE |
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