The Japanese Society for Regenerative Medicine

Autor: Shinichiro Kobayashi, Tota Kugiyama, Takanobu Hara, Takashi Hamada, Susumu Eguchi, Masaaki Hidaka, Yasuhiro Maruya, Kyoichiro Maekawa, Mitsuhisa Takatsuki, Yusuke Sakai, Akihiko Soyama, Shinichiro Ono, Tomohiko Adachi, Satomi Okada, Toshiyuki Adachi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
BAS
biliary anastomotic strictures
Pathology
medicine.medical_specialty
medicine.medical_treatment
Biomedical Engineering
Anastomotic healing
Adipose tissue
Adipose-derived stem cell
MSC
mesenchymal stem cell
Anastomosis
CBD
common bile duct
Biomaterials
03 medical and health sciences
0302 clinical medicine
APC
allophycocyanin
FBS
fetal bovine serum
Laparotomy
medicine
lcsh:QH573-671
lcsh:R5-920
business.industry
Bile duct
lcsh:Cytology
BMSC
bone marrow stem cells
medicine.disease
VEGF
vascular endothelial growth factor
FGF
fibroblast growth factor
Transplantation
030104 developmental biology
medicine.anatomical_structure
ADSC
adipose-derived stem cell
Original Article
HGF
hepatocyte growth factor
Biliary anastomosis
Stem cell
business
Wound healing
lcsh:Medicine (General)
Infiltration (medical)
030217 neurology & neurosurgery
Cell sheet
Developmental Biology
Zdroj: Regenerative Therapy
Regenerative Therapy, Vol 14, Iss, Pp 79-86 (2020)
ISSN: 2352-3204
Popis: Introduction: Cell sheets consisting of adipose-derived stem cells (ADSCs) have been reported to be effective for wound healing. We conducted this study to clarify the efficacy of ADSC sheets in wound healing at the duct-to-duct biliary anastomotic site in pigs. Methods: Eleven female pigs (20?25 kg) were divided into two groups: biliary anastomosis with an ADSC sheet (n = 6) or without an ADSC sheet (n = 5). To follow the transplanted ADSCs, PKH26GL-labeled sheets were used in one of the ADSC pigs. Two weeks prior to laparotomy, ADSCs were isolated from the lower abdominal subcutaneous adipose tissue. After three passages, ADSCs were seeded on temperature-responsive culture dishes and collected as cell sheets. ADSC sheets were gently transplanted on the anastomotic site. We evaluated specimens by PKH26GL labeling, macroscopic changes, infiltration of inflammatory cells, and collagen content. Results: Labeled ADSCs remained around the bile duct wall. In the no-ADSC group, more adhesion developed at the hepatic hilum as observed during relaparotomy. Histopathological examination showed that the diameter and cross-sectional area of the bile duct wall were decreased in the ADSC group. In the no-ADSC group, a large number of inflammatory cells and more collagen fibers were identified in the bile duct wall. Conclusions: The present study demonstrated that autologous ADSC sheet transplantation reduced hypertrophic changes in the bile duct wall at the anastomotic site. A long-term follow-up is required to evaluate the efficacy of this mechanism in prevention of biliary anastomotic strictures.
Regenerative Therapy, 14, pp.79-86; 2020
Databáze: OpenAIRE
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