Deciphering the genome of polyphosphate accumulating actinobacterium Microlunatus phosphovorus
| Autor: | Natsuko Ichikawa, Junji Fukada, Yoichi Kamagata, Akatsuki Kawakoshi, Yoko Katano, Sanae Nakamura, Shuji Yamazaki, Hidekazu Nakazawa, Nobuyuki Fujita, Satoshi Hanada, Machi Sasagawa, Akira Hosoyama, Kazunori Nakamura, Hiroki Sasaki |
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| Rok vydání: | 2012 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Biology Genome Polyhydroxyalkanoates Microbiology Actinobacteria Polyphosphates Genetics Phosphate Transport Proteins Molecular Biology Gene Phylogeny Exopolyphosphatase Base Sequence Phosphotransferases whole genome sequence polyphosphate accumulating organism polyhydroxyalkanoate polyphosphate General Medicine Sequence Analysis DNA Propionibacteriaceae Full Papers biology.organism_classification Physical Chromosome Mapping Acid Anhydride Hydrolases Microlunatus phosphovorus Polyphosphate-accumulating organisms Biochemistry bacteria Proteobacteria Genome Bacterial |
| Zdroj: | DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1756-1663 |
| Popis: | Polyphosphate accumulating organisms (PAOs) belong mostly to Proteobacteria and Actinobacteria and are quite divergent. Under aerobic conditions, they accumulate intracellular polyphosphate (polyP), while they typically synthesize polyhydroxyalkanoates (PHAs) under anaerobic conditions. Many ecological, physiological, and genomic analyses have been performed with proteobacterial PAOs, but few with actinobacterial PAOs. In this study, the whole genome sequence of an actinobacterial PAO, Microlunatus phosphovorus NM-1(T) (NBRC 101784(T)), was determined. The number of genes for polyP metabolism was greater in M. phosphovorus than in other actinobacteria; it possesses genes for four polyP kinases (ppks), two polyP-dependent glucokinases (ppgks), and three phosphate transporters (pits). In contrast, it harbours only a single ppx gene for exopolyphosphatase, although two copies of ppx are generally present in other actinobacteria. Furthermore, M. phosphovorus lacks the phaABC genes for PHA synthesis and the actP gene encoding an acetate/H(+) symporter, both of which play crucial roles in anaerobic PHA accumulation in proteobacterial PAOs. Thus, while the general features of M. phosphovorus regarding aerobic polyP accumulation are similar to those of proteobacterial PAOs, its anaerobic polyP use and PHA synthesis appear to be different. |
| Databáze: | OpenAIRE |
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