Membrane antigens of human bronchial epithelial cells identified by monoclonal antibodies
| Autor: | Samuel D. Bernal, Yuk-Chor Wong, Carl O'Hara, K. Weinberg, M. Kakefuda, Rolf A. Stahel |
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| Rok vydání: | 1988 |
| Předmět: |
medicine.drug_class
Clinical Biochemistry Cell Fluorescent Antibody Technique Bronchi Plant Science Columnar Cell Cross Reactions Monoclonal antibody Epithelium Immunoenzyme Techniques Antigen immune system diseases hemic and lymphatic diseases medicine Humans Cells Cultured biology Immunoperoxidase Antibodies Monoclonal Epithelial Cells Cell Biology bacterial infections and mycoses Molecular biology medicine.anatomical_structure Cell culture Antigens Surface Immunology biology.protein lipids (amino acids peptides and proteins) Antibody Biotechnology Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology. 24:117-125 |
| ISSN: | 1475-2689 0883-8364 |
| DOI: | 10.1007/bf02623888 |
| Popis: | Subpopulations of normal bronchial epithelial cells were identified using a series of murine monoclonal antibodies. These antibodies were used to stain intact bronchial epithelial cells in culture by indirect immunofluorescence. LAM 2 reacted with 80%, LAM 6 with 75%, LAM 7 with 60%, and LAM 8 with 5% of these cells. Sections of human bronchial epithelium were also stained with these antibodies by immunoperoxidase methods. LAM 2 was found to bind with 80%, LAM 6 with 65%, LAM 7 with 50%, and LAM 8 with less than 1% of bronchial epithelial cells. LAM 2 stained both columnar epithelial cells and basal cells; LAM 6 stained mainly basal cells and only a small proportion of columnar cells; LAM 7 showed specificity for basal cells; LAM 8 distinctly stained single cells in the basal cell layer. These antibodies were previously shown to react with the surface membrane of human lung carcinomas, ranging from the broad reactivity of LAM 2 with small cell and non-small cell lung cancers to the highly restricted reactivity of LAM 8 with small cell carcinomas of the lung. Thus, membrane antigens have been identified in bronchial epithelial cells by monoclonal antibodies which exhibit a similar range of cellular reactivity in vitro as in vivo. Inasmuch as these antibodies recognize subsets of cells which could not be easily distinguished by morphologic characteristics, these reagents may be useful in classifying bronchial epithelial cells. |
| Databáze: | OpenAIRE |
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