Glycolysis and methylaminoisobutyrate uptake in rat-1 cells transfected with ras or myc oncogenes
| Autor: | R J Resnick, R Feldman, E Racker |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Aminoisobutyric Acids
Platelet-derived growth factor Biological Transport Active Biology Transfection chemistry.chemical_compound Methionine Animals Glycolysis Cycloheximide Platelet-Derived Growth Factor Multidisciplinary Dose-Response Relationship Drug Oncogene Genetic transfer Oncogenes Fibroblasts Molecular biology Rats Cell Transformation Neoplastic chemistry Biochemistry Anaerobic glycolysis Cell culture Transforming Growth Factors Peptides Research Article Transforming growth factor |
| Zdroj: | Proceedings of the National Academy of Sciences. 82:3535-3538 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.82.11.3535 |
| Popis: | A high rate of aerobic glycolysis was catalyzed by rat-1 cells transfected with a ras oncogene (ras cells); rat-1 cells and rat-1 cells transfected with myc oncogene (myc cells) showed a low rate of glycolysis that was increased after exposure of the cells to type B transforming growth factor (TGF-beta). The uptake of radioactive methylaminoisobutyric acid or L-methionine via system A of amino acid transport also was accelerated after exposure of these cells to TGF-beta, with the myc cells being most sensitive and the ras cells least sensitive. Methionine was found to be a potent inhibitor of glycolysis in ras cells as well as in rat-1 or myc cells that were exposed to TGF-beta. We propose a relationship between the product of the ras oncogene (p21) and the protein(s) induced by exposure to TGF-beta. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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