The effect of divalent cations on the thermostability of type II polyketide synthase acyl carrier proteins
| Autor: | Joris Beld, Bradley Studnitzer, Kristen E. Fiore, Valentine C. Courouble, Louise K. Charkoudian, Miranda C. Baker, Kerilyn N. Godbe, Kavita D. Shroff, Karl A. Johnson, David L. Cookmeyer, Ashley C. Sisto, Adam R. Huff, Stephen T. Ridings, Keisuke Sawada, Aurelio Mollo, Saadia Nawal, Michael R. Jordan, Marco A. Rivas, Emily N. Krasnow, Robert Fairman, Alexander J. Frost, Bashkim Kokona, Grace A. R. Thiele |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Environmental Engineering Stereochemistry General Chemical Engineering Sequence (biology) Article Divalent 03 medical and health sciences stomatognathic system Denaturation (biochemistry) Thermostability chemistry.chemical_classification 030102 biochemistry & molecular biology biology ATP synthase humanities Acyl carrier protein 030104 developmental biology chemistry Helix biology.protein bacteria lipids (amino acids peptides and proteins) Function (biology) Biotechnology |
| Zdroj: | AIChE Journal. 64:4308-4318 |
| ISSN: | 1547-5905 0001-1541 |
| Popis: | The successful engineering of biosynthetic pathways hinges on understanding the factors that influence acyl carrier protein (ACP) stability and function. The stability and structure of ACPs can be influenced by the presence of divalent cations, but how this relates to primary sequence remains poorly understood. As part of a course-based undergraduate research experience, we investigated the thermostability of type II polyketide synthase (PKS) ACPs. We observed an approximate 40 °C range in the thermostability amongst the 14 ACPs studied, as well as an increase in stability (5 – 26 °C) of the ACPs in the presence of divalent cations. Distribution of charges in the helix II-loop-helix III region was found to impact the enthalpy of denaturation. Taken together, our results reveal clues as to how the sequence of type II PKS ACPs relates to their structural stability, information that can be used to study how ACP sequence relates to function. |
| Databáze: | OpenAIRE |
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