Selective method for identification and quantification of Bifidobacterium animalis subspecies lactis BB-12 (BB-12) from the gastrointestinal tract of healthy volunteers ingesting a combination probiotic of BB-12 and Lactobacillus rhamnosus GG
| Autor: | I J Mahoney, Anne Kane, Julie McKinney, Rina Leyva, Cheleste M. Thorpe, Debra D. Poutsiaka, Lisa Davidson, L. A. McDermott, Barry R. Goldin, David R. Snydman, L Lathrop Stern, C Baez-Giangreco |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Bifidobacterium animalis subspecies lactis Adult Male food.ingredient Adolescent 030106 microbiology Applied Microbiology and Biotechnology Microbiology law.invention 03 medical and health sciences Probiotic Feces Young Adult food Lactobacillus rhamnosus Bifidobacterium animalis law Healthy volunteers Ingestion Agar Humans Food science Aged Aged 80 and over Gastrointestinal tract biology Lacticaseibacillus rhamnosus Probiotics General Medicine Middle Aged Tetracycline biology.organism_classification Healthy Volunteers Anti-Bacterial Agents Gastrointestinal Tract 030104 developmental biology Dietary Supplements Female Biotechnology |
| Zdroj: | Journal of applied microbiology. 122(5) |
| ISSN: | 1365-2672 |
| Popis: | Aim To develop a novel validated method for the isolation of Bifidobacterium animalis ssp. lactis BB-12 (BB-12) from faecal specimens and apply it to studies of BB-12 and Lactobacillus rhamnosus GG (LGG) recovered from the healthy human gastrointestinal (GI) tract. Methods and Results A novel method for isolating and enumerating BB-12 was developed based on its morphologic features of growth on tetracycline-containing agar. The method identified BB-12 correctly from spiked stool close to 100% of the time as validated by PCR confirmation of identity, and resulted in 97–104% recovery of BB-12. The method was then applied in a study of the recovery of BB-12 and LGG from the GI tract of healthy humans consuming ProNutrients® Probiotic powder sachet containing BB-12 and LGG. Viable BB-12 and LGG were recovered from stool after 21 days of probiotic ingestion compared to baseline. In contrast, no organisms were recovered 21 days after baseline in the nonsupplemented control group. Conclusions We demonstrated recovery of viable BB-12, using a validated novel method specific for the isolation of BB-12, and LGG from the GI tract of healthy humans who consumed the probiotic supplement. Significance and Impact of the Study This method will enable more detailed and specific studies of BB-12 in probiotic supplements, including when in combination with LGG. |
| Databáze: | OpenAIRE |
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