FSE–Ag complex NS: preparation and evaluation of antibacterial activity
Autor: | Pradeep Kumar Bolla, Sai H.S. Boddu, Jwala Renukuntla, Rahul S. Kalhapure, Amit Dahal, Delfina C. Dominguez |
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Rok vydání: | 2018 |
Předmět: |
Staphylococcus aureus
Silver Dispersity Metal Nanoparticles Microbial Sensitivity Tests 02 engineering and technology 030226 pharmacology & pharmacy Nanocomposites 03 medical and health sciences 0302 clinical medicine Anti-Infective Agents Dynamic light scattering Furosemide Escherichia coli Zeta potential Electrical and Electronic Engineering Solubility Chemistry Broth microdilution 021001 nanoscience & nanotechnology Antimicrobial Electronic Optical and Magnetic Materials Pseudomonas aeruginosa Microscopy Electron Scanning Particle size 0210 nano-technology Antibacterial activity Research Article Biotechnology Nuclear chemistry |
Zdroj: | IET Nanobiotechnol |
ISSN: | 1751-875X 1751-8741 |
Popis: | Silver (Ag) complexes of drugs and their nanosystems have great potential as antibacterials. Recently, an Ag complex of furosemide (Ag–FSE) has shown to be a promising antimicrobial. However, poor solubility of Ag–FSE could hamper its introduction into clinics. Therefore, the authors developed a nanosuspension of Ag–FSE (Ag–FSE_NS) for its solubility and antibacterial activity enhancement. The aim of this study was to introduce a novel nanoantibiotic with enhanced antibacterial efficacy. Ag–FSE_NS was prepared by precipitation–ultrasonication technique. Size, polydispersity index (PI) and zeta potential (ZP) of prepared Ag–FSE_NS were measured by dynamic light scattering, whereas surface morphology was determined using scanning electron microscopy (SEM). In vitro antibacterial activity was evaluated against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using broth microdilution method. Size, PI and ZP of optimised Ag–FSE_NS1 were 191.2 ± 19.34 nm, 0.465 ± 0.059 and −55.7 ± 8.18 mV, respectively. SEM revealed that Ag–FSE_NS1 particles were rod or needle‐like with smooth surfaces. Saturation solubility of Ag–FSE in NS increased eight‐fold than pure Ag–FSE. Ag–FSE_NS1 exhibited two‐fold and eight‐fold enhancements in activity against E. coli and S. aureus, respectively. The results obtained showed that developed Ag–FSE_NS1 holds a promise as a topical antibacterial. |
Databáze: | OpenAIRE |
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