Geraniin induces apoptotic cell death in human lung adenocarcinoma A549 cells in vitro and in vivo
| Autor: | Jimei Yin, Siran Wang, Linna Pan, Jia Li |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Lung Neoplasms
Physiology Geraniin Mice Nude Adenocarcinoma of Lung Apoptosis Adenocarcinoma Biology S Phase Mice chemistry.chemical_compound Glucosides In vivo Cell Line Tumor Physiology (medical) Animals Humans Cell Proliferation bcl-2-Associated X Protein Membrane Potential Mitochondrial Pharmacology A549 cell Cell Death Caspase 3 Cytochrome c Cytochromes c Cell Cycle Checkpoints General Medicine Cell cycle Molecular biology Caspase 9 Hydrolyzable Tannins Mitochondria Cell biology Proto-Oncogene Proteins c-bcl-2 chemistry Cell culture Cancer cell biology.protein Reactive Oxygen Species |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 91:1016-1024 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/cjpp-2013-0140 |
| Popis: | Geraniin has previously been reported to possess extensive biological activity. In this study, we reported that geraniin is an inhibitor of tumor activity in vitro and in vivo. Geraniin suppressed the proliferation of A549 cells in a dose- and time-dependent manner. Geraniin arrested the cell cycle in the S phase and induced a significant accumulation of reactive oxygen species (ROS), as well as an increased percentage of cells with mitochondrial membrane potential (MMP) disruption. Western blot analysis showed that geraniin inhibited Bcl-2 expression and induced Bax expression to disintegrate the outer mitochondrial membrane and cause cytochrome c release. Mitochondrial cytochrome c release was associated with the activation of caspase-9 and caspase-3 cascades. Additionally, geraniin resulted in tumor growth inhibition in A549 xenografts. Our results indicate cytotoxic activity of geraniin towards cancer cells in vitro and in vivo. |
| Databáze: | OpenAIRE |
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