GABA expression and regulation by sensory experience in the developing visual system
| Autor: | Loïs S. Miraucourt, Jianli Li, Jorge Santos da Silva, Kasandra Burgos, Hikari Abe, Hollis T. Cline, Edward S. Ruthazer |
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| Rok vydání: | 2011 |
| Předmět: |
Superior Colliculi
Xenopus Sensation lcsh:Medicine Fluorescent Antibody Technique Stimulation Sensory system Enzyme-Linked Immunosorbent Assay Biology gamma-Aminobutyric acid Retina 03 medical and health sciences Xenopus laevis 0302 clinical medicine Model Organisms Developmental Neuroscience Molecular Cell Biology medicine Neuropil Animals Visual Pathways GABAergic Neurons lcsh:Science gamma-Aminobutyric Acid 030304 developmental biology 0303 health sciences Multidisciplinary Superior colliculus lcsh:R Brain Anatomy Animal Models biology.organism_classification Sensory Systems Visual cortex medicine.anatomical_structure nervous system Larva Cellular Neuroscience GABAergic lcsh:Q Cellular Types Neuroscience 030217 neurology & neurosurgery Photic Stimulation medicine.drug Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 1, p e29086 (2012) |
| ISSN: | 1932-6203 |
| Popis: | The developing retinotectal system of the Xenopus laevis tadpole is a model of choice for studying visual experience-dependent circuit maturation in the intact animal. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to play a critical role in the formation of sensory circuits in this preparation, however a comprehensive neuroanatomical study of GABAergic cell distribution in the developing tadpole has not been conducted. We report a detailed description of the spatial expression of GABA immunoreactivity in the Xenopus laevis tadpole brain at two key developmental stages: stage 40/42 around the onset of retinotectal innervation and stage 47 when the retinotectal circuit supports visually-guided behavior. During this period, GABAergic neurons within specific brain structures appeared to redistribute from clusters of neuronal somata to a sparser, more uniform distribution. Furthermore, we found that GABA levels were regulated by recent sensory experience. Both ELISA measurements of GABA concentration and quantitative analysis of GABA immunoreactivity in tissue sections from the optic tectum show that GABA increased in response to a 4 hr period of enhanced visual stimulation in stage 47 tadpoles. These observations reveal a remarkable degree of adaptability of GABAergic neurons in the developing brain, consistent with their key contributions to circuit development and function. |
| Databáze: | OpenAIRE |
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