LncRNA THEMIS2-211, a tumor-originated circulating exosomal biomarker, promotes the growth and metastasis of hepatocellular carcinoma by functioning as a competing endogenous RNA
| Autor: | Jiyou Yao, Xuefeng Hua, Jiewei Shi, Xiaoyuan Hu, Kayin Lui, Kaitao He, Jialuo Mai, Tian Lan, Minqiang Lu |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
Carcinoma Hepatocellular Epithelial-Mesenchymal Transition Liver Neoplasms Intracellular Signaling Peptides and Proteins Mice Nude Apoptosis Biochemistry Xenograft Model Antitumor Assays Gene Expression Regulation Neoplastic Mice Genetics Biomarkers Tumor Disease Progression Tumor Cells Cultured Animals Humans Proteoglycans RNA Long Noncoding Molecular Biology Biotechnology Cell Proliferation |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 36(4) |
| ISSN: | 1530-6860 |
| Popis: | Hepatocellular carcinoma (HCC) is a major challenge for human health. Finding reliable diagnostic biomarkers and therapeutic targets for HCC is highly desired in the clinic. Currently, circulating exosomal lncRNA is a promising biomarker for the diagnosis of cancer and lncRNA is also a potential target in cancer therapy. Here, the diagnostic value of a panel based on exosomal lncRNA THEMIS2-211 and PRKACA-202, superior to that of AFP, was identified for diagnosing human HCC. Besides, the performance of exosomal lncRNA THEMIS2-211 alone exceeds that of AFP in diagnosing early-stage HCC patients (stage I). Furthermore, lncRNA THEMIS2-211 is highly expressed in HCC tissues and correlated with the poor prognosis of HCC patients. LncRNA THEMIS2-211 is upregulated and localized in the cytoplasm of HCC cells. LncRNA THEMIS2-211 exerts its biological function as an oncogene that promotes the proliferation, migration, invasion, EMT of HCC cells by physically interacting with miR-940 and therefore promoting SPOCK1 expressions. Rescue assays show the regulation of SPOCK1 by lncRNA THEMIS2-211 dependents on miR-940. The discovery of lncRNA THEMIS2-211 further illuminates the molecular pathogenesis of HCC and the THEMIS2-211/miR-940/SPOCK1 axis may act as a potential therapeutic target for HCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text