Genetic variation in the VP7 gene of rotavirus G1P[8] strains isolated in Vietnam, 1998–2009
| Autor: | Dang Duc Anh, Vu T. B. Hau, Le Kim Anh, Le Thi Luan, Nguyen Van Trang, Tetsu Yamashiro |
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| Rok vydání: | 2012 |
| Předmět: |
Rotavirus
Cancer Research viruses Lineage (evolution) Molecular Sequence Data Biology medicine.disease_cause Rotavirus Infections fluids and secretions Phylogenetics Virology Genotype Genetic variation medicine Cluster Analysis Humans Antigens Viral Phylogeny Genetics Molecular Epidemiology Phylogenetic tree Molecular epidemiology Infant Newborn Genetic Variation Infant virus diseases Sequence Analysis DNA Rotavirus vaccine Gastroenteritis Infectious Diseases Vietnam Child Preschool DNA Viral Capsid Proteins |
| Zdroj: | Virus Research. 165:190-196 |
| ISSN: | 0168-1702 |
| Popis: | Group A rotavirus genotype G1P[8] is the most common strain affecting humans around the world over the past few decades. In this study, we examined genetic variation in the VP7 gene of rotavirus G1P[8] strains, detected in children of four major cities of Vietnam during three different rotavirus seasons: 1998-1999, 2007-2008 and 2008-2009 in order to assess the evolution of the virus over 11 years. Fecal samples (n=73) from children hospitalized for gastroenteritis caused by G1P[8] rotavirus were analyzed by DNA sequencing of gene 9 encoding the VP7 capsid protein. Phylogenetic analyses indicated that VP7 gene of the G1 strains from 1999 contained a lineage I, while rotaviruses from 2009 clustered in lineage II. Both of these lineages were found co-circulating in 2007-2008 season. While different sublineages of lineage I and II co-circulated in the 1998-1999 and 2007-2008 seasons, almost all strains in 2009 belonged to sub-lineage II-C. In the analysis using selected 10 strains, the VP4 genes of these 2 VP7-G1 lineages were all grouped in F45-like cluster. Deduced amino acid analyses indicated that there were thirteen amino acid substitutions between strains of two lineages. Of those, two were found in antigenic regions A and C, implying possible antigenic differences between these two lineages. The G1P[8] strains in Vietnam are very genetically diverse and dynamic, implying the frequent monitoring on evolution of rotavirus will be important to assess efficacy of rotavirus vaccine in Vietnam. |
| Databáze: | OpenAIRE |
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