High-speed, three-dimensional imaging reveals chemotactic behaviour specific to human-infective Leishmania parasites
| Autor: | Laurence G. Wilson, Kirstin A Spence, Rachel C Findlay, Pegine B. Walrad, Mohamed Osman, Paul M. Kaye |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Science Leishmania mexicana Motility Stimulus (physiology) Flagellum Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine image analysis chemotaxis Biology (General) optical microscopy General Immunology and Microbiology General Neuroscience Chemotaxis General Medicine Leishmania biology.organism_classification Phenotype Cell biology 030104 developmental biology Medicine Adaptation 030217 neurology & neurosurgery |
| Zdroj: | eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| Popis: | Cellular motility is an ancient eukaryotic trait, ubiquitous across phyla with roles in predator avoidance, resource access, and competition. Flagellar motility is seen in various parasitic protozoans, and morphological changes in flagella during the parasite life cycle have been observed. We studied the impact of these changes on motility across life cycle stages, and how such changes might serve to facilitate human infection. We used holographic microscopy to image swimming cells of different Leishmania mexicana life cycle stages in three dimensions. We find that the human-infective (metacyclic promastigote) forms display ‘run and tumble’ behaviour in the absence of stimulus, reminiscent of bacterial motion, and that they specifically modify swimming direction and speed to target host immune cells in response to a macrophage-derived stimulus. Non-infective (procyclic promastigote) cells swim more slowly, along meandering helical paths. These findings demonstrate adaptation of swimming phenotype and chemotaxis towards human cells. |
| Databáze: | OpenAIRE |
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