Proteomics-based investigation in C2C12 myoblast differentiation
| Autor: | Sabrina Burattini, Lucia Casadei, Luciana Vallorani, Anna Maria Gioacchini, Elisabetta Falcieri, Vilberto Stocchi, Michele Guescini, A. D’Emilio, L. Biagiotti |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Proteomics
Histology C2C12 cells differentiation proteome TEM 2-D electrophoresis mass spectrometry mass spectrometry QH301-705.5 Cellular differentiation 2-D electrophoresis proteome Biophysics Biology Muscle Development Mass Spectrometry Cell Line Myoblasts Mice C2C12 cells differentiation TEM Skeletal muscle cell differentiation Microscopy Electron Transmission Heat shock protein tem Animals Myocyte Electrophoresis Gel Two-Dimensional Biology (General) lcsh:QH301-705.5 Heat-Shock Proteins Myogenesis Cell Differentiation Brief Note Cell Biology 2-d electrophoresis Cell biology lcsh:Biology (General) Proteome c2c12 cells C2C12 |
| Zdroj: | European Journal of Histochemistry, Vol 53, Iss 4, Pp 261-268 (2009) Scopus-Elsevier European Journal of Histochemistry, Vol 53, Iss 4, Pp e31-e31 (2009) European Journal of Histochemistry : EJH |
| Popis: | Skeletal muscle cell differentiation is a multistage process extensively studied over the years. Even if great improvements have been achieved in defining biological process underlying myogenesis, many molecular mechanisms need still to be clarified. To further highlight this process, we studied cells at undifferentiated, intermediate and highly differentiated stages, and we analyzed, for each condition, morphological and proteomic changes. We also identified the proteins that showed statistical significant changes by a ESI-Q-TOF mass spectrometer. This work provides further evidence of the involvement of particular proteins in skeletal muscle development. Furthermore, the high level of expression of many heat shock proteins, suggests a relationship between differentiation and cellular stress. Intriguingly, the discovery of myogenesis-correlated proteins, known to play a role in apoptosis, suggests a link between differentiation and this type of cell death. |
| Databáze: | OpenAIRE |
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