Recruitment of monocytes and mature macrophages in mouse pubic symphysis relaxation during pregnancy and postpartum recovery†
| Autor: | Bianca Gazieri Castelucci, Paulo Pinto Joazeiro, Viviane Souza Rosa, Sílvio Roberto Consonni |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Offspring Macrophage polarization Pubic symphysis Biology Monocytes Proinflammatory cytokine Andrology 03 medical and health sciences Mice 0302 clinical medicine Pregnancy Gene expression medicine Macrophage Animals Postpartum Recovery mouse Ligaments pubic symphysis Macrophages Postpartum Period Cell Biology General Medicine medicine.disease 030104 developmental biology medicine.anatomical_structure Reproductive Medicine 030220 oncology & carcinogenesis tissue remodeling Female recruited macrophage Research Article |
| Zdroj: | Biology of Reproduction |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Appropriate remodeling of the female lower reproductive tract and pelvic floor is essential during normal mammalian pregnancy, labor, and postpartum recovery. During mouse pregnancy, in addition to reproductive tract modifications, the pubic symphysis (PS) is remodeled into a soft interpubic ligament (IpL) to provide safe delivery of the offspring and fast postpartum recovery. Although temporal changes in the phenotypes of myeloid cells, such as mononuclear phagocytes, are crucial to remodeling the lower reproductive tract organs in preparation for a safe delivery, little is known about the involvement of recruited monocytes or macrophages in mouse PS remodeling. We used combined light microscopy, electron microscopy, and qPCR analysis to investigate the profile of recruited monocytes and macrophage polarization markers in C57Bl6 mouse interpubic tissues during pregnancy (D12, D18, and D19) and early days postpartum (1 dpp and 3 dpp) to better identify their presence in proper remodeling of the mouse PS. Our morphological data show that the number of recruited monocytes is increased in interpubic tissues and that recruited monocytes differentiate into proinflammatory or anti-inflammatory macrophage phenotypes from D18 to 3 dpp, which may contribute to dynamic changes in the gene expression of specific inflammatory mediators involved in interpubic tissue remodeling at these time points. Therefore, our morphological and quantitative gene expression data suggest that both differentiated macrophages from recruited monocytes and polarized macrophages may collaborate for IpL relaxation at labor and the appropriate repair of the PS after the first pregnancy. Recruited monocytes and mature macrophages are present in the mouse pubic symphysis and may contribute to mouse pubic symphysis relaxation during late pregnancy and postpartum recovery. |
| Databáze: | OpenAIRE |
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