Factors influencing the acute pentylenetetrazole-induced seizure paradigm and a literature review

Autor:	Karenna J Groff, Achint Singh, Mustafa Sahin, Sameer C. Dhamne, Leigh-Ana Rossitto, Bo Zhang, Lahin K Lalani, Alexander Rotenberg, Christopher J. Yuskaitis
Rok vydání:	2021
Předmět:	0301 basic medicine Male medicine.medical_specialty Multivariate analysis Mice 129 Strain Neurosciences. Biological psychiatry. Neuropsychiatry Convulsants Logistic regression 03 medical and health sciences Epilepsy Mice 0302 clinical medicine Sex Factors Species Specificity Seizures Internal medicine medicine Animals Pooled data RC346-429 Research Articles Critical gap business.industry General Neuroscience Critical factors Age Factors Electroencephalography Odds ratio medicine.disease Confidence interval Mice Inbred C57BL 030104 developmental biology Social Isolation Mice Inbred CBA Pentylenetetrazole Female Neurology (clinical) Neurology. Diseases of the nervous system business 030217 neurology & neurosurgery RC321-571 Research Article
Zdroj:	Annals of Clinical and Translational Neurology Annals of Clinical and Translational Neurology, Vol 8, Iss 7, Pp 1388-1397 (2021)
ISSN:	2328-9503
Popis:	Objective To confirm the critical factors affecting seizure susceptibility in acute pentylenetetrazole (PTZ) mouse epilepsy models and evaluate the prior literature for these factors. Methods Serial cohorts of wild‐type mice administered intraperitoneal (IP)‐PTZ were aggregated and analyzed by multivariate logistic regression for the effect of sex, age, background strain, dose, and physiologic stress (i.e., EEG implantation and/or single‐housing) on seizure response. We assessed the reporting of these factors in a comprehensive literature review over the last 10 years (2010–2020). Results We conducted aggregated analysis of pooled data of 307 mice (220 C57BL/6J mice and 87 mixed background mice; 202 males, 105 females) with median age of 10 weeks (range: 6–49 weeks) with acute PTZ injection (dose range 40–65 mg/kg). Significance in multivariate analysis was found between seizures and increased PTZ dose (odds ratio (OR) 1.149, 95% confidence interval (CI) 1.102–1.205), older age (OR 1.1, 95% CI 1.041–1.170), physiologic stress (OR 17.36, 95% CI 7.349–44.48), and mixed background strain (OR 0.4725, 95% CI 0.2315–0.9345). Literature review identified 97 papers using acute PTZ‐seizure models. Age, housing, sex, and background were omitted by 61% (59/97), 51% (49/97), 18% (17/97), and 8% (8/97) papers, respectively. Only 17% of publications specified all four factors (16/97). Interpretation Our analysis and literature review demonstrate a critical gap in standardization of acute PTZ‐induced seizure paradigm in mice. We recommend that future studies specify and control for age, background strain, sex, and housing conditions of experimental animals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ab621acb90ad0973fb781ba48ab5f15 https://pubmed.ncbi.nlm.nih.gov/34102033 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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